Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 188599..189133 Replicon plasmid pE1_230
Accession NZ_CP018066
Organism Enterococcus faecium strain E1

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag BO233_RS16320 Protein ID WP_002360769.1
Coordinates 188599..188874 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag BO233_RS16325 Protein ID WP_002369771.1
Coordinates 188867..189133 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BO233_RS16285 183796..184524 - 729 WP_002368105.1 hypothetical protein -
BO233_RS16295 185174..186433 - 1260 WP_002368103.1 PcfJ domain-containing protein -
BO233_RS16300 186433..187212 - 780 WP_002368102.1 hypothetical protein -
BO233_RS16305 187443..187589 - 147 WP_002368101.1 DpnD/PcfM family protein -
BO233_RS16310 187611..187796 - 186 WP_002368099.1 hypothetical protein -
BO233_RS16320 188599..188874 - 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
BO233_RS16325 188867..189133 - 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BO233_RS16330 189244..189828 - 585 WP_002368095.1 thermonuclease family protein -
BO233_RS16335 189852..190268 - 417 WP_002368093.1 single-stranded DNA-binding protein -
BO233_RS16340 190366..190860 - 495 WP_002368092.1 DnaJ domain-containing protein -
BO233_RS16345 190893..191159 - 267 WP_002368091.1 hypothetical protein -
BO233_RS16355 191751..192239 - 489 WP_002368088.1 hypothetical protein -
BO233_RS16360 192245..193030 - 786 WP_000803546.1 replication-relaxation family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / ant(6)-Ia / erm(B) - 1..230049 230049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T68659 WP_002360769.1 NZ_CP018066:c188874-188599 [Enterococcus faecium]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T68659 NZ_CP018066:c188874-188599 [Enterococcus faecium]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT68659 WP_002369771.1 NZ_CP018066:c189133-188867 [Enterococcus faecium]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT68659 NZ_CP018066:c189133-188867 [Enterococcus faecium]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References