Detailed information of TA system TA068658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 149443..150112
• Replicon: plasmid pE1_230
• Accession: NZ_CP018066
• Organism: Enterococcus faecium strain E1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: BO233_RS16050
• Protein ID: WP_010708525.1
• Coordinates: 149443..149838 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: BO233_RS16055
• Protein ID: WP_002331065.1
• Coordinates: 149840..150112 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BO233_RS16015	145269..145902	-	634	Protein_150	peptidase	-
BO233_RS16020	146162..146710	-	549	WP_002292681.1	hypothetical protein	-
BO233_RS16025	146711..147568	-	858	WP_002292680.1	AAA family ATPase	-
BO233_RS16030	147854..148141	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BO233_RS16035	148131..148460	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
BO233_RS16040	148537..148683	-	147	Protein_155	Txe/YoeB family addiction module toxin	-
BO233_RS16045	148735..149415	-	681	WP_061086363.1	IS6-like element IS1216 family transposase	-
BO233_RS16050	149443..149838	-	396	WP_010708525.1	zeta toxin family protein	Toxin
BO233_RS16055	149840..150112	-	273	WP_002331065.1	antitoxin	Antitoxin
BO233_RS16060	150129..150338	-	210	WP_000527318.1	peptide-binding protein	-
BO233_RS16065	150437..151333	-	897	WP_002334978.1	ParA family protein	-
BO233_RS16080	153051..153623	-	573	WP_001079938.1	HTH domain-containing protein	-
BO233_RS16090	153899..154693	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	-	1..230049	230049
	-	inside	IScluster/Tn	aph(3')-III / ant(6)-Ia / erm(B)	-	148735..160229	11494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T68658 WP_010708525.1 NZ_CP018066:c149838-149443 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

>T68658 NZ_CP018066:c149838-149443 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT68658 WP_002331065.1 NZ_CP018066:c150112-149840 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT68658 NZ_CP018066:c150112-149840 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55