Detailed information of TA system TA068657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 39385..39992
• Replicon: plasmid pE1_230
• Accession: NZ_CP018066
• Organism: Enterococcus faecium strain E1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: BO233_RS15390
• Protein ID: WP_002321032.1
• Coordinates: 39642..39992 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: BO233_RS15385
• Protein ID: WP_002287514.1
• Coordinates: 39385..39648 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BO233_RS15340	34421..35320	+	900	WP_010733696.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BO233_RS15345	35323..35820	+	498	WP_048946612.1	single-stranded DNA-binding protein	-
BO233_RS15350	36186..36446	+	261	WP_002296240.1	hypothetical protein	-
BO233_RS15355	36891..37097	+	207	WP_002325537.1	hypothetical protein	-
BO233_RS15360	37145..37348	+	204	WP_110300627.1	hypothetical protein	-
BO233_RS15365	37364..37801	+	438	WP_002332738.1	hypothetical protein	-
BO233_RS15370	37794..38501	+	708	WP_002332739.1	hypothetical protein	-
BO233_RS15380	38881..39060	+	180	WP_002305761.1	hypothetical protein	-
BO233_RS15385	39385..39648	+	264	WP_002287514.1	hypothetical protein	Antitoxin
BO233_RS15390	39642..39992	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BO233_RS15395	40016..40630	-	615	WP_002318230.1	recombinase family protein	-
BO233_RS15400	40944..41366	+	423	WP_002288787.1	hypothetical protein	-
BO233_RS15405	41376..41579	+	204	WP_002299573.1	hypothetical protein	-
BO233_RS15410	41790..42374	+	585	WP_002299575.1	recombinase family protein	-
BO233_RS15415	42658..43338	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
BO233_RS15425	43657..44529	-	873	WP_002305879.1	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	-	1..230049	230049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T68657 WP_002321032.1 NZ_CP018066:39642-39992 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T68657 NZ_CP018066:39642-39992 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT68657 WP_002287514.1 NZ_CP018066:39385-39648 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT68657 NZ_CP018066:39385-39648 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0