Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1500271..1500931 Replicon chromosome
Accession NZ_CP018061
Organism Enterococcus mundtii strain DSM 4838

Toxin (Protein)


Gene name hicA Uniprot ID A0A2K8TH03
Locus tag EM4838_RS07185 Protein ID WP_071868107.1
Coordinates 1500740..1500931 (-) Length 64 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2K8TF28
Locus tag EM4838_RS07180 Protein ID WP_071868108.1
Coordinates 1500271..1500705 (-) Length 145 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EM4838_RS07150 1496517..1497326 - 810 WP_071867466.1 propanediol utilization microcompartment protein PduB -
EM4838_RS07155 1497336..1497617 - 282 WP_071867467.1 BMC domain-containing protein -
EM4838_RS07160 1497898..1498806 + 909 WP_071867468.1 PocR ligand-binding domain-containing protein -
EM4838_RS07165 1499175..1499609 - 435 WP_071867469.1 EutP/PduV family microcompartment system protein -
EM4838_RS07170 1499653..1500006 - 354 WP_071867470.1 BMC domain-containing protein -
EM4838_RS07180 1500271..1500705 - 435 WP_071868108.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EM4838_RS07185 1500740..1500931 - 192 WP_071868107.1 type II toxin-antitoxin system HicA family toxin Toxin
EM4838_RS07190 1501194..1501601 - 408 WP_100917255.1 NusG domain II-containing protein -
EM4838_RS07195 1501638..1502015 - 378 WP_071867699.1 hypothetical protein -
EM4838_RS07200 1502047..1502214 - 168 WP_071867698.1 50S ribosomal protein L32 -
EM4838_RS07205 1502232..1502501 - 270 WP_065095743.1 30S ribosomal protein S14 -
EM4838_RS07210 1502753..1503112 - 360 WP_071867697.1 YxeA family protein -
EM4838_RS07215 1503354..1504715 - 1362 WP_071867696.1 C39 family peptidase -
EM4838_RS07220 1504942..1505649 - 708 WP_071867695.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-62)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(10-104)


Sequences

Toxin        


Download         Length: 64 a.a.        Molecular weight: 7285.47 Da        Isoelectric Point: 10.7055

>T68651 WP_071868107.1 NZ_CP018061:c1500931-1500740 [Enterococcus mundtii]
MPLTGKEMLRLLKKNGWIERRQEGSHHHHHLYKDGVRITVPVHANQDLGRGLEQKILKDAGLK

Download         Length: 192 bp

>T68651 NZ_CP018061:c1500931-1500740 [Enterococcus mundtii]
ATGCCGCTTACAGGAAAAGAAATGCTTCGGTTATTGAAGAAGAATGGTTGGATTGAGCGAAGGCAGGAAGGCTCTCATCA
TCATCATCACCTTTATAAAGATGGCGTTAGAATTACTGTCCCTGTTCATGCCAATCAAGATCTAGGCCGAGGACTTGAGC
AAAAAATTTTGAAGGATGCGGGATTGAAATAA

Antitoxin


Download         Length: 145 a.a.        Molecular weight: 16125.32 Da        Isoelectric Point: 4.3064

>AT68651 WP_071868108.1 NZ_CP018061:c1500705-1500271 [Enterococcus mundtii]
MLSEPLAKTYPAIFKPEEGGGYFIEFPDIQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEEQIPEPTPIKEVFAEKD
SFTTLIRVDVAKYLKDTELVKKTLTIPRWADTLGKRAGINFSVLLTESIADKADDILHSEKNNR

Download         Length: 435 bp

>AT68651 NZ_CP018061:c1500705-1500271 [Enterococcus mundtii]
ATGTTAAGCGAACCTTTAGCAAAAACATATCCTGCTATTTTTAAACCCGAAGAAGGTGGCGGTTACTTCATTGAATTTCC
TGATATTCAGGGTGCTTATACAGGAATCAATGAAGATGATATCTCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTCTAGCGGACTATATTGAGCACGAAGAGCAAATACCAGAGCCAACGCCGATCAAAGAAGTCTTTGCTGAAAAAGAT
TCCTTTACGACATTGATTCGTGTAGATGTTGCTAAATATCTAAAGGATACAGAACTTGTCAAAAAGACTTTAACGATACC
ACGATGGGCCGATACGTTAGGCAAACGTGCGGGGATAAATTTTTCAGTCCTTTTAACAGAATCCATTGCAGATAAAGCGG
ATGATATTCTTCATTCAGAGAAAAATAATAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K8TH03

Antitoxin

Source ID Structure
AlphaFold DB A0A2K8TF28

References