Detailed information of TA system TA068651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1500271..1500931
• Replicon: chromosome
• Accession: NZ_CP018061
• Organism: Enterococcus mundtii strain DSM 4838

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2K8TH03
• Locus tag: EM4838_RS07185
• Protein ID: WP_071868107.1
• Coordinates: 1500740..1500931 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2K8TF28
• Locus tag: EM4838_RS07180
• Protein ID: WP_071868108.1
• Coordinates: 1500271..1500705 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EM4838_RS07150	1496517..1497326	-	810	WP_071867466.1	propanediol utilization microcompartment protein PduB	-
EM4838_RS07155	1497336..1497617	-	282	WP_071867467.1	BMC domain-containing protein	-
EM4838_RS07160	1497898..1498806	+	909	WP_071867468.1	PocR ligand-binding domain-containing protein	-
EM4838_RS07165	1499175..1499609	-	435	WP_071867469.1	EutP/PduV family microcompartment system protein	-
EM4838_RS07170	1499653..1500006	-	354	WP_071867470.1	BMC domain-containing protein	-
EM4838_RS07180	1500271..1500705	-	435	WP_071868108.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EM4838_RS07185	1500740..1500931	-	192	WP_071868107.1	type II toxin-antitoxin system HicA family toxin	Toxin
EM4838_RS07190	1501194..1501601	-	408	WP_100917255.1	NusG domain II-containing protein	-
EM4838_RS07195	1501638..1502015	-	378	WP_071867699.1	hypothetical protein	-
EM4838_RS07200	1502047..1502214	-	168	WP_071867698.1	50S ribosomal protein L32	-
EM4838_RS07205	1502232..1502501	-	270	WP_065095743.1	30S ribosomal protein S14	-
EM4838_RS07210	1502753..1503112	-	360	WP_071867697.1	YxeA family protein	-
EM4838_RS07215	1503354..1504715	-	1362	WP_071867696.1	C39 family peptidase	-
EM4838_RS07220	1504942..1505649	-	708	WP_071867695.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7285.47 Da
• Isoelectric Point: 10.7055

>T68651 WP_071868107.1 NZ_CP018061:c1500931-1500740 [Enterococcus mundtii]
MPLTGKEMLRLLKKNGWIERRQEGSHHHHHLYKDGVRITVPVHANQDLGRGLEQKILKDAGLK

Nucleotide

• Length: 192 bp

>T68651 NZ_CP018061:c1500931-1500740 [Enterococcus mundtii]
ATGCCGCTTACAGGAAAAGAAATGCTTCGGTTATTGAAGAAGAATGGTTGGATTGAGCGAAGGCAGGAAGGCTCTCATCA
TCATCATCACCTTTATAAAGATGGCGTTAGAATTACTGTCCCTGTTCATGCCAATCAAGATCTAGGCCGAGGACTTGAGC
AAAAAATTTTGAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16125.32 Da
• Isoelectric Point: 4.3064

>AT68651 WP_071868108.1 NZ_CP018061:c1500705-1500271 [Enterococcus mundtii]
MLSEPLAKTYPAIFKPEEGGGYFIEFPDIQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEEQIPEPTPIKEVFAEKD
SFTTLIRVDVAKYLKDTELVKKTLTIPRWADTLGKRAGINFSVLLTESIADKADDILHSEKNNR

Nucleotide

• Length: 435 bp

>AT68651 NZ_CP018061:c1500705-1500271 [Enterococcus mundtii]
ATGTTAAGCGAACCTTTAGCAAAAACATATCCTGCTATTTTTAAACCCGAAGAAGGTGGCGGTTACTTCATTGAATTTCC
TGATATTCAGGGTGCTTATACAGGAATCAATGAAGATGATATCTCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTCTAGCGGACTATATTGAGCACGAAGAGCAAATACCAGAGCCAACGCCGATCAAAGAAGTCTTTGCTGAAAAAGAT
TCCTTTACGACATTGATTCGTGTAGATGTTGCTAAATATCTAAAGGATACAGAACTTGTCAAAAAGACTTTAACGATACC
ACGATGGGCCGATACGTTAGGCAAACGTGCGGGGATAAATTTTTCAGTCCTTTTAACAGAATCCATTGCAGATAAAGCGG
ATGATATTCTTCATTCAGAGAAAAATAATAGATAA

Structures

Toxin

• AlphaFold DB: A0A2K8TH03

Antitoxin

• AlphaFold DB: A0A2K8TF28