Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 780377..781062 Replicon chromosome
Accession NZ_CP018058
Organism Geobacillus thermocatenulatus strain KCTC 3921

Toxin (Protein)


Gene name mazF Uniprot ID A0A0J0V7Q9
Locus tag GT3921_RS03980 Protein ID WP_025949892.1
Coordinates 780377..780763 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0J0V7L9
Locus tag GT3921_RS03985 Protein ID WP_025949893.1
Coordinates 780763..781062 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT3921_RS03955 775420..776190 + 771 WP_025949888.1 hypothetical protein -
GT3921_RS19060 776481..776600 - 120 Protein_725 transposase -
GT3921_RS03960 776641..776880 - 240 WP_075261378.1 DUF4277 domain-containing protein -
GT3921_RS03965 776940..778010 + 1071 WP_025949890.1 hypothetical protein -
GT3921_RS03970 778380..779879 + 1500 WP_025949891.1 carboxylesterase/lipase family protein -
GT3921_RS03975 779912..780127 - 216 WP_011232518.1 hypothetical protein -
GT3921_RS03980 780377..780763 - 387 WP_025949892.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GT3921_RS03985 780763..781062 - 300 WP_025949893.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GT3921_RS03990 781247..781483 + 237 WP_013144265.1 preprotein translocase subunit SecG -
GT3921_RS03995 781558..782298 + 741 WP_025949894.1 carboxylesterase -
GT3921_RS04000 782321..784597 + 2277 WP_025949895.1 ribonuclease R -
GT3921_RS04005 784679..785146 + 468 WP_011232513.1 SsrA-binding protein SmpB -
GT3921_RS19290 785161..785316 + 156 WP_167372761.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-118)

Antitoxin

SpoVT-AbrB domain

(23-68)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14112.30 Da        Isoelectric Point: 5.6642

>T68647 WP_025949892.1 NZ_CP018058:c780763-780377 [Geobacillus thermocatenulatus]
MPADVPERGDFVVLNFHPQAGHEQAGRRIAIVLSPKKFNQATGFVAVCPITNQKKGYPFEVDLPKGGILLEGGGDPITGV
ILADQIKSLDWKARHLKVLKKYNPEDAQMEEIDEIIDECLAKIATYLT

Download         Length: 387 bp

>T68647 NZ_CP018058:c780763-780377 [Geobacillus thermocatenulatus]
ATGCCCGCAGACGTACCGGAAAGAGGAGATTTCGTTGTGTTGAACTTCCATCCACAAGCTGGCCATGAACAGGCAGGAAG
AAGAATTGCCATTGTTTTATCTCCTAAAAAATTCAATCAGGCAACAGGATTTGTGGCTGTTTGTCCAATCACCAATCAGA
AGAAAGGCTATCCGTTTGAAGTGGATTTGCCAAAAGGAGGAATACTCCTTGAAGGCGGCGGCGATCCCATCACAGGAGTC
ATTTTAGCGGATCAAATCAAATCATTGGATTGGAAAGCCCGTCATCTAAAAGTTTTAAAAAAATACAATCCGGAAGATGC
TCAAATGGAGGAAATCGATGAAATCATTGACGAATGCCTGGCAAAAATCGCAACGTATCTGACTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11316.89 Da        Isoelectric Point: 6.4778

>AT68647 WP_025949893.1 NZ_CP018058:c781062-780763 [Geobacillus thermocatenulatus]
MNSKQNNIAKRKGENDMSTVIVQKWGNSLGIRIPKDAADQIGIQQGSEMELHVIENEGIITLKPKRTRKKYTLEELISQI
TPENRHEEMDFGVEGRELI

Download         Length: 300 bp

>AT68647 NZ_CP018058:c781062-780763 [Geobacillus thermocatenulatus]
ATGAATTCAAAACAAAACAACATCGCAAAAAGGAAAGGAGAGAATGATATGTCTACCGTAATCGTGCAAAAGTGGGGGAA
CAGTTTGGGCATTCGCATCCCAAAAGACGCGGCTGACCAAATCGGGATTCAACAAGGTTCTGAAATGGAATTACATGTCA
TTGAAAACGAAGGAATCATCACATTAAAGCCGAAAAGAACACGGAAGAAATACACACTGGAAGAACTGATATCACAAATT
ACCCCTGAAAATCGACATGAGGAGATGGATTTCGGGGTAGAAGGACGTGAGTTGATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J0V7Q9

Antitoxin

Source ID Structure
AlphaFold DB A0A0J0V7L9

References