Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 47762..48398 Replicon chromosome
Accession NZ_CP018058
Organism Geobacillus thermocatenulatus strain KCTC 3921

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GT3921_RS00270 Protein ID WP_003253417.1
Coordinates 47762..48112 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0D8C733
Locus tag GT3921_RS00275 Protein ID WP_013144044.1
Coordinates 48117..48398 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT3921_RS00250 42998..44135 + 1138 Protein_32 IS110 family transposase -
GT3921_RS00255 44591..45109 - 519 WP_025950325.1 SprT family protein -
GT3921_RS00260 45198..45311 + 114 WP_013144046.1 cortex morphogenetic protein CmpA -
GT3921_RS00265 45330..47495 - 2166 WP_047752393.1 RNA-binding transcriptional accessory protein -
GT3921_RS00270 47762..48112 - 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GT3921_RS00275 48117..48398 - 282 WP_013144044.1 hypothetical protein Antitoxin
GT3921_RS00280 48522..49676 - 1155 WP_025950327.1 alanine racemase -
GT3921_RS00285 49925..50938 - 1014 WP_025950328.1 outer membrane lipoprotein carrier protein LolA -
GT3921_RS00290 51018..51407 - 390 WP_025950329.1 holo-ACP synthase -
GT3921_RS00295 51521..52138 + 618 WP_089113999.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 43548..44135 587


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T68646 WP_003253417.1 NZ_CP018058:c48112-47762 [Geobacillus thermocatenulatus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T68646 NZ_CP018058:c48112-47762 [Geobacillus thermocatenulatus]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGACTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10669.17 Da        Isoelectric Point: 7.2027

>AT68646 WP_013144044.1 NZ_CP018058:c48398-48117 [Geobacillus thermocatenulatus]
VSESGATTEIVVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT68646 NZ_CP018058:c48398-48117 [Geobacillus thermocatenulatus]
GTGTCGGAATCTGGCGCAACAACGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAATGGCAACCGCAATGAACTCATTTATCAGGCGACGAAAATGTACATTCGCGAGCGGAAAAAGCGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8C733

References