Detailed information of TA system TA068626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 78546..80299
• Replicon: chromosome
• Accession: NZ_CP018055
• Organism: Burkholderia pseudomallei strain VB976100

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0F6L1Z5
• Locus tag: BK015_RS21300
• Protein ID: WP_004542147.1
• Coordinates: 78546..79898 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BK015_RS21305
• Protein ID: WP_071811030.1
• Coordinates: 79895..80299 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK015_RS21275	74871..75212	-	342	WP_004539420.1	hypothetical protein	-
BK015_RS21280	75489..76442	-	954	WP_004539421.1	agmatinase	-
BK015_RS21285	76609..77523	+	915	WP_004198844.1	LysR family transcriptional regulator	-
BK015_RS40820	77690..77836	+	147	WP_004199790.1	hypothetical protein	-
BK015_RS21295	77937..78389	+	453	WP_024428481.1	hypothetical protein	-
BK015_RS21300	78546..79898	-	1353	WP_004542147.1	type II toxin-antitoxin system HipA family toxin	Toxin
BK015_RS21305	79895..80299	-	405	WP_071811030.1	helix-turn-helix domain-containing protein	Antitoxin
BK015_RS40825	80637..80900	-	264	WP_004199789.1	hypothetical protein	-
BK015_RS21325	81366..83744	+	2379	WP_071811475.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
BK015_RS21330	83749..85269	+	1521	WP_071811032.1	cellulase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49818.85 Da
• Isoelectric Point: 9.8300

>T68626 WP_004542147.1 NZ_CP018055:c79898-78546 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHVLADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T68626 NZ_CP018055:c79898-78546 [Burkholderia pseudomallei]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGTGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCATCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCACGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14306.62 Da
• Isoelectric Point: 11.7233

>AT68626 WP_071811030.1 NZ_CP018055:c80299-79895 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLANRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARAGARRAAPPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT68626 NZ_CP018055:c80299-79895 [Burkholderia pseudomallei]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
GTTGACGCAGGCGATGCTCGCGAACCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGCGCGGGCGCGCGACGTGCCGCGCCGCCCGC
GTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCCCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACT
GGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L1Z5