Detailed information of TA system TA068623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2348655..2349256
• Replicon: chromosome
• Accession: NZ_CP018049
• Organism: Pseudomonas orientalis strain F9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BOP93_RS10750
• Protein ID: WP_104502582.1
• Coordinates: 2349005..2349256 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1B5E0X2
• Locus tag: BOP93_RS10745
• Protein ID: WP_065894293.1
• Coordinates: 2348655..2349008 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOP93_RS10725	2344009..2344479	+	471	WP_104502579.1	hypothetical protein	-
BOP93_RS27675	2344649..2345902	+	1254	WP_205885798.1	hypothetical protein	-
BOP93_RS10735	2346087..2347442	+	1356	WP_104502580.1	MATE family efflux transporter	-
BOP93_RS10740	2347483..2348676	+	1194	WP_104502581.1	MFS transporter	-
BOP93_RS10745	2348655..2349008	-	354	WP_065894293.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BOP93_RS10750	2349005..2349256	-	252	WP_104502582.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9501.99 Da
• Isoelectric Point: 10.0448

>T68623 WP_104502582.1 NZ_CP018049:c2349256-2349005 [Pseudomonas orientalis]
VSKNEKLLAKLLDEHVAFTWSERVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T68623 NZ_CP018049:c2349256-2349005 [Pseudomonas orientalis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCGATGAGCACGTGGCATTCACCTGGTCTGAACGCGTAACGCTGTT
ACGCCGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGACCCCAGTGCACTGATTA
CCCTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 12765.63 Da
• Isoelectric Point: 7.3242

>AT68623 WP_065894293.1 NZ_CP018049:c2349008-2348655 [Pseudomonas orientalis]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCPAIG

Nucleotide

• Length: 354 bp

>AT68623 NZ_CP018049:c2349008-2348655 [Pseudomonas orientalis]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGTCTGTTCGGCAAAAT
CCTGTTCATCAAGGCGTTGGTGAGCTACGAAGGCAAAACCGTCGCCGAGCTGGACGCGGCCTTCAGAGAGGCGGTGGACG
ATTACCTCGCCACCTGCCAGGCCCTCGGGCAAACGCCGGAAAAGCCTTGCAAGGGCTCGTTCAATGTACGCGTGGGCCAT
GACCTGCATTTGGCGGCAGCGCTGGCGGCGACCCGCAAGAAAGTGACGTTGAACGATCTGACACGCCAGGCGCTGAATGA
GTTTTTACAACATCATTGCCCGGCGATTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B5E0X2