Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1902203..1902807 Replicon chromosome
Accession NZ_CP018049
Organism Pseudomonas orientalis strain F9

Toxin (Protein)


Gene name hicA Uniprot ID A0A2N1E473
Locus tag BOP93_RS08660 Protein ID WP_065934378.1
Coordinates 1902203..1902391 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1B5E431
Locus tag BOP93_RS08665 Protein ID WP_065894766.1
Coordinates 1902403..1902807 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOP93_RS08630 1897937..1898473 + 537 WP_104502276.1 DsbE family thiol:disulfide interchange protein -
BOP93_RS08635 1898470..1898943 + 474 WP_104502277.1 cytochrome c-type biogenesis protein CcmH -
BOP93_RS08640 1898936..1900120 + 1185 WP_104502278.1 c-type cytochrome biogenesis protein CcmI -
BOP93_RS08645 1900136..1900531 + 396 WP_065898045.1 hypothetical protein -
BOP93_RS08650 1900610..1900885 - 276 WP_104502279.1 DUF3077 domain-containing protein -
BOP93_RS08655 1901508..1902074 + 567 WP_104502280.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
BOP93_RS08660 1902203..1902391 + 189 WP_065934378.1 type II toxin-antitoxin system HicA family toxin Toxin
BOP93_RS08665 1902403..1902807 + 405 WP_065894766.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BOP93_RS08670 1902804..1904000 - 1197 WP_104502281.1 MFS transporter -
BOP93_RS08675 1904241..1905245 - 1005 WP_065894770.1 sulfate ABC transporter substrate-binding protein -
BOP93_RS08680 1905341..1906165 - 825 WP_104502282.1 ion transporter -
BOP93_RS08685 1906197..1907072 - 876 WP_104502283.1 urea transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7204.45 Da        Isoelectric Point: 11.2000

>T68622 WP_065934378.1 NZ_CP018049:1902203-1902391 [Pseudomonas orientalis]
VQSRLLIKELLEAGWVLDRVTGSHHLFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Download         Length: 189 bp

>T68622 NZ_CP018049:1902203-1902391 [Pseudomonas orientalis]
GTGCAAAGCAGGCTATTGATCAAGGAGTTGCTGGAAGCGGGCTGGGTGCTGGATCGGGTTACCGGCAGCCATCACCTTTT
CACCCACCGCTATAACCCGTACACGATACCGGTGCCCCATCCGAAGAAGGATCTGCCGTTGGGCACTGTGAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.62 Da        Isoelectric Point: 4.4765

>AT68622 WP_065894766.1 NZ_CP018049:1902403-1902807 [Pseudomonas orientalis]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHLMLQEIAAEGGVIPMPTSVATHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT68622 NZ_CP018049:1902403-1902807 [Pseudomonas orientalis]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGCGCCGT
CACCGCCGGGGACAGTTTCGAAGAGGCCTACAACGCGGCCGTGGAAGTGGCGCACCTCATGCTGCAGGAGATTGCCGCAG
AGGGCGGGGTGATTCCAATGCCAACCTCGGTTGCCACTCATCACGCCCATGAAGACTACGCCGGTATGGGCTGGGGCATG
TTGGAGCTGGATATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGTTATGTGATCCAACGCAT
CGACCGTTACGTGCGTGAACACAACGTCAAAAGCCGCTCGTCATTTCTGGCGGATGCGGCTTTGGAGAAGTTGGTGCGTT
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N1E473

Antitoxin

Source ID Structure
AlphaFold DB A0A1B5E431

References