Detailed information of TA system TA068621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1646060..1646576
• Replicon: chromosome
• Accession: NZ_CP018049
• Organism: Pseudomonas orientalis strain F9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BOP93_RS07365
• Protein ID: WP_104502102.1
• Coordinates: 1646060..1646347 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BOP93_RS07370
• Protein ID: WP_057723048.1
• Coordinates: 1646337..1646576 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOP93_RS07345	1641061..1641909	-	849	WP_104502098.1	N-6 DNA methylase	-
BOP93_RS27385	1642111..1642413	-	303	WP_157943480.1	hypothetical protein	-
BOP93_RS07350	1642623..1643036	-	414	WP_104502099.1	hypothetical protein	-
BOP93_RS07355	1643050..1643868	-	819	WP_104502100.1	ParA family protein	-
BOP93_RS07360	1644222..1645799	-	1578	WP_157943481.1	tyrosine-type recombinase/integrase	-
BOP93_RS07365	1646060..1646347	-	288	WP_104502102.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BOP93_RS07370	1646337..1646576	-	240	WP_057723048.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BOP93_RS07380	1647255..1648481	-	1227	WP_104502103.1	MFS transporter	-
BOP93_RS07385	1648727..1650652	-	1926	WP_065886283.1	response regulator	-
BOP93_RS07390	1650642..1651505	-	864	WP_065886284.1	sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11211.92 Da
• Isoelectric Point: 9.4193

>T68621 WP_104502102.1 NZ_CP018049:c1646347-1646060 [Pseudomonas orientalis]
MTYSLDFDACALKEWQKFGDTLRQQLKKKLVEILNTPRNEANRLHGLPDCYKIKLRSSGYRLIYQVIDQEITVVVVAVDK
REHDQAYRKVNERLS

Nucleotide

• Length: 288 bp

>T68621 NZ_CP018049:c1646347-1646060 [Pseudomonas orientalis]
ATGACTTATAGCCTTGATTTTGATGCATGCGCATTGAAGGAATGGCAAAAATTCGGCGACACCCTGCGCCAGCAGCTCAA
GAAGAAGCTGGTGGAGATTCTGAACACCCCCCGCAACGAAGCCAATCGCCTGCATGGACTCCCTGACTGCTACAAGATCA
AATTACGCAGTAGCGGCTACAGACTGATCTATCAAGTGATCGATCAGGAAATTACCGTCGTCGTGGTGGCCGTGGATAAG
CGGGAGCATGATCAGGCCTATCGCAAGGTGAATGAGCGCTTAAGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8973.39 Da
• Isoelectric Point: 4.6017

>AT68621 WP_057723048.1 NZ_CP018049:c1646576-1646337 [Pseudomonas orientalis]
MASINVRIDDDLKARAYLELEKLGVTPSELMRQALQYVAERGQLPFKPVLMTEEDEALMATVRERLASPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT68621 NZ_CP018049:c1646576-1646337 [Pseudomonas orientalis]
ATGGCATCTATCAACGTTCGTATTGATGACGACCTGAAAGCGCGCGCCTATCTCGAATTGGAGAAGCTCGGCGTCACGCC
CTCCGAGCTGATGCGTCAGGCGCTGCAGTACGTAGCCGAGCGTGGTCAGCTGCCATTCAAGCCCGTGCTGATGACGGAGG
AAGATGAAGCCTTGATGGCCACCGTACGTGAACGCTTGGCATCTCCCCAGCGAGTGAAGGTTTCGTTGGATGACTTATAG