Detailed information of TA system TA068595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1158165..1158806
• Replicon: chromosome
• Accession: NZ_CP018034
• Organism: Glaesserella parasuis str. Nagasaki

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B8F4G6
• Locus tag: A2U21_RS06155
• Protein ID: WP_012621798.1
• Coordinates: 1158624..1158806 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A2U21_RS06150
• Protein ID: WP_021109631.1
• Coordinates: 1158165..1158584 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U21_RS06115	1153279..1153923	-	645	WP_035498099.1	antirepressor	-
A2U21_RS06120	1154171..1155196	-	1026	WP_021109600.1	hypothetical protein	-
A2U21_RS06125	1155528..1156061	-	534	WP_010786726.1	hypothetical protein	-
A2U21_RS06130	1156317..1156559	-	243	WP_005713710.1	hypothetical protein	-
A2U21_RS06135	1156903..1157124	-	222	WP_020997039.1	hypothetical protein	-
A2U21_RS06140	1157139..1157324	+	186	WP_020997221.1	YegP family protein	-
A2U21_RS06145	1157350..1157637	-	288	WP_012621801.1	hypothetical protein	-
A2U21_RS06150	1158165..1158584	-	420	WP_021109631.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A2U21_RS06155	1158624..1158806	-	183	WP_012621798.1	type II toxin-antitoxin system HicA family toxin	Toxin
A2U21_RS06160	1158927..1159718	-	792	WP_021109630.1	DUF1828 domain-containing protein	-
A2U21_RS06165	1159718..1160209	-	492	WP_021109629.1	hypothetical protein	-
A2U21_RS06170	1160261..1160926	-	666	WP_021109628.1	helix-turn-helix transcriptional regulator	-
A2U21_RS06175	1161071..1161286	+	216	WP_035500508.1	helix-turn-helix domain-containing protein	-
A2U21_RS06185	1161566..1162348	+	783	WP_021109626.1	Rha family transcriptional regulator	-
A2U21_RS06190	1162345..1163175	+	831	WP_035497995.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1129538..1190159	60621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6883.01 Da
• Isoelectric Point: 10.2994

>T68595 WP_012621798.1 NZ_CP018034:c1158806-1158624 [Glaesserella parasuis str. Nagasaki]
MKYSEFLRYLLAQGCEIETQKRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T68595 NZ_CP018034:c1158806-1158624 [Glaesserella parasuis str. Nagasaki]
ATGAAATACAGCGAGTTCCTAAGATACTTACTTGCACAAGGTTGCGAAATCGAAACACAAAAGCGAGGAAGCCATCGAAA
GGTGACACTAAACGGCAAACAATCTGTTTTCCCATATCACGGCAGTAAAGAGATTGGTACAGGTTTAGTGAATAAAATTA
AAAAAGACTTAGATCTTAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16051.53 Da
• Isoelectric Point: 5.2662

>AT68595 WP_021109631.1 NZ_CP018034:c1158584-1158165 [Glaesserella parasuis str. Nagasaki]
MLRYPVEITKDDNNTYLVTCPDIPEMASVGEDIEEALLEAKDGLAAALEFYFDDRRTIPLPSPVKEGQHTVDLSILQSMK
VFLLNEMIKQDVRKAEMARRLDVHLPQIDRLLDFKHATKVEFVEKAYSKLNRHFTILPH

Nucleotide

• Length: 420 bp

>AT68595 NZ_CP018034:c1158584-1158165 [Glaesserella parasuis str. Nagasaki]
ATGTTACGTTACCCCGTAGAAATCACCAAAGATGACAATAATACCTACTTAGTGACTTGTCCGGATATTCCCGAAATGGC
AAGCGTTGGCGAAGATATTGAAGAAGCATTACTTGAAGCAAAAGATGGCTTAGCGGCAGCTCTTGAATTTTATTTTGATG
ATAGACGCACCATTCCATTACCAAGCCCTGTAAAAGAGGGACAACACACCGTCGATTTATCTATTCTTCAATCAATGAAA
GTGTTTTTACTCAATGAAATGATTAAACAAGATGTACGAAAAGCAGAAATGGCAAGACGTTTAGATGTACACCTACCACA
AATTGACCGCTTGCTGGATTTTAAGCACGCAACCAAAGTAGAATTTGTCGAGAAAGCTTATAGTAAACTCAATCGCCACT
TTACTATTCTCCCCCACTAG

Structures

Toxin

• AlphaFold DB: B8F4G6