Detailed information of TA system TA068593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 943669..944291
• Replicon: chromosome
• Accession: NZ_CP018034
• Organism: Glaesserella parasuis str. Nagasaki

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A836YYE8
• Locus tag: A2U21_RS04875
• Protein ID: WP_005715063.1
• Coordinates: 944109..944291 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A837B0B6
• Locus tag: A2U21_RS04870
• Protein ID: WP_005715065.1
• Coordinates: 943669..944082 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U21_RS04835	939535..940218	-	684	WP_021109615.1	helix-turn-helix transcriptional regulator	-
A2U21_RS04840	940349..940549	+	201	WP_016527563.1	helix-turn-helix domain-containing protein	-
A2U21_RS04845	940570..940845	+	276	WP_021109614.1	bacteriophage CII family protein	-
A2U21_RS04850	940829..941611	+	783	WP_021109626.1	Rha family transcriptional regulator	-
A2U21_RS04855	941608..942438	+	831	WP_035497995.1	hypothetical protein	-
A2U21_RS04860	942438..943112	+	675	WP_051502669.1	hypothetical protein	-
A2U21_RS04865	943207..943635	+	429	WP_035498003.1	recombination protein NinB	-
A2U21_RS04870	943669..944082	-	414	WP_005715065.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A2U21_RS04875	944109..944291	-	183	WP_005715063.1	type II toxin-antitoxin system HicA family toxin	Toxin
A2U21_RS04880	944538..945107	+	570	WP_035497972.1	recombination protein NinG	-
A2U21_RS04885	945209..946417	+	1209	WP_021109463.1	IS256 family transposase	-
A2U21_RS04890	946424..946804	+	381	WP_035498288.1	antitermination protein	-
A2U21_RS04900	946989..948197	-	1209	WP_035498286.1	IS256 family transposase	-
A2U21_RS04905	948245..948586	-	342	Protein_911	type II toxin-antitoxin system HicB family antitoxin	-
A2U21_RS04910	948616..948801	-	186	WP_005714898.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	930112..1019391	89279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-130)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6678.80 Da
• Isoelectric Point: 10.6602

>T68593 WP_005715063.1 NZ_CP018034:c944291-944109 [Glaesserella parasuis str. Nagasaki]
VDSKTIIKMIEADGWYLVATSGSHHQFKHPTKQGRVTVPHPKKDLKKGTELSILKQAGLK

Nucleotide

• Length: 183 bp

>T68593 NZ_CP018034:c944291-944109 [Glaesserella parasuis str. Nagasaki]
GTGGATAGCAAAACCATAATTAAAATGATTGAAGCGGACGGTTGGTACTTAGTTGCAACAAGTGGAAGCCACCATCAATT
CAAACATCCAACTAAACAAGGGCGAGTAACAGTGCCTCATCCGAAAAAGGATTTAAAAAAAGGAACTGAACTCTCAATAC
TTAAACAAGCGGGGCTAAAATAG

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15401.47 Da
• Isoelectric Point: 4.3988

>AT68593 WP_005715065.1 NZ_CP018034:c944082-943669 [Glaesserella parasuis str. Nagasaki]
MLYPIAIEPGDETHAYGVIVPDIPGCFSAGDTLEEAYQNVKEAIVFHLEGLVEDGEEIPMPTSIDNHRKNPDFAEYDFFF
GFVDVDISHLLGKAERINITLPSYLIKRIDDFVAVHKEYKNRSNFLAKIAADKILTA

Nucleotide

• Length: 414 bp

>AT68593 NZ_CP018034:c944082-943669 [Glaesserella parasuis str. Nagasaki]
ATGTTATACCCAATTGCAATTGAACCAGGTGACGAAACTCACGCTTACGGCGTTATCGTGCCTGATATTCCAGGGTGCTT
CTCTGCTGGCGATACCCTCGAAGAAGCATATCAAAATGTCAAAGAAGCCATCGTGTTCCACCTTGAAGGCTTAGTTGAAG
ATGGAGAAGAAATTCCGATGCCAACCTCCATTGATAACCACCGCAAAAACCCTGATTTTGCAGAATATGACTTCTTTTTC
GGCTTTGTTGATGTTGATATTAGCCATTTACTCGGCAAAGCCGAACGCATCAACATCACCCTCCCTAGCTATCTCATCAA
ACGCATTGATGATTTTGTTGCCGTTCACAAAGAGTATAAAAACCGTAGCAACTTCTTAGCTAAAATCGCAGCGGATAAAA
TCTTAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A836YYE8

Antitoxin

• AlphaFold DB: A0A837B0B6