Detailed information of TA system TA068590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 132869..134418
• Replicon: chromosome
• Accession: NZ_CP018034
• Organism: Glaesserella parasuis str. Nagasaki

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: A2U21_RS00580
• Protein ID: WP_021110129.1
• Coordinates: 132869..134185 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A836YYU2
• Locus tag: A2U21_RS00585
• Protein ID: WP_005713287.1
• Coordinates: 134179..134418 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U21_RS00535	127968..128555	+	588	WP_020457627.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
A2U21_RS00540	128811..128990	-	180	Protein_101	disulfide bond formation protein DsbA	-
A2U21_RS00545	128990..129433	-	444	WP_020457625.1	nuclear transport factor 2 family protein	-
A2U21_RS00550	129568..129684	+	117	Protein_103	LysR family transcriptional regulator	-
A2U21_RS00555	129765..130338	-	574	Protein_104	virulence RhuM family protein	-
A2U21_RS00560	130525..130884	-	360	WP_010787368.1	hypothetical protein	-
A2U21_RS00565	130784..131299	+	516	WP_080691048.1	hypothetical protein	-
A2U21_RS00570	131323..132084	+	762	WP_021110131.1	hypothetical protein	-
A2U21_RS00575	132118..132696	+	579	WP_021110130.1	PLP-dependent aminotransferase family protein	-
A2U21_RS00580	132869..134185	-	1317	WP_021110129.1	type II toxin-antitoxin system HipA family toxin	Toxin
A2U21_RS00585	134179..134418	-	240	WP_005713287.1	helix-turn-helix domain-containing protein	Antitoxin
A2U21_RS00590	134566..135393	-	828	WP_021110128.1	alpha/beta hydrolase	-
A2U21_RS00595	135524..136969	+	1446	WP_021110127.1	metalloprotease TldD	-
A2U21_RS00600	137224..137634	+	411	WP_005713295.1	preprotein translocase subunit SecE	-
A2U21_RS00605	137636..138214	+	579	WP_005713298.1	transcription termination/antitermination protein NusG	-
A2U21_RS00610	138340..138594	-	255	WP_005713300.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49974.19 Da
• Isoelectric Point: 7.3465

>T68590 WP_021110129.1 NZ_CP018034:c134185-132869 [Glaesserella parasuis str. Nagasaki]
MVMRLRVLMNGEMVGYFDRTLSGSHHFSYAESWLNSPLARPISLSMPLQRATYQGKEVINFFDNLLPDNPQIRARIATRF
NAETQQPFDLLAKIGRDAAGAITLLGEDEQIPNHRIIQATELDESEMTKILQSYQYNAPLGMIDNNQDFRISIAGAQEKT
ALLWHNGKWKLPHGLTPTTHIFKLPMGEIRTPKHLLDLSKSVDNELVCLRLFSAFNIPSANVELLEMNDIRALAVERFDR
RLSQDGSYFLRLPYEDFCQAFGLSPTQKYESDGGIGIGDMMNKLLYSSNSNDRRQFMQTQFLFWLLAAIDGHAKNFSLAI
EKQGRYHLSPVYDVLSAYPMLGGKGWHHSELQMAMSLRSTQKGKKWHWSKIYAEHFYATAKFVGFSSSDMKQVIEQICDT
VQETIDSVATTLPKQIDENVRDQIFTGIMKQVQKVSMK

Nucleotide

• Length: 1317 bp

>T68590 NZ_CP018034:c134185-132869 [Glaesserella parasuis str. Nagasaki]
ATGGTAATGCGATTACGAGTATTAATGAATGGCGAAATGGTTGGGTATTTTGACCGCACTTTATCGGGTTCCCACCATTT
TTCCTATGCTGAAAGTTGGCTAAATTCCCCATTAGCTCGCCCGATTTCCCTTTCTATGCCCCTGCAAAGAGCAACATATC
AAGGCAAAGAAGTGATTAATTTCTTTGATAATTTACTGCCCGATAATCCTCAAATTCGAGCAAGAATTGCGACAAGATTT
AATGCTGAAACACAACAACCTTTTGACTTACTTGCTAAAATTGGTCGCGATGCTGCGGGTGCTATTACTCTTTTAGGGGA
AGATGAACAAATTCCTAATCATCGAATAATTCAAGCCACAGAATTAGACGAAAGTGAAATGACGAAAATTCTACAAAGTT
ATCAATACAATGCCCCACTTGGTATGATTGATAATAATCAGGACTTCAGAATTTCTATTGCTGGTGCACAAGAAAAGACA
GCTCTGCTTTGGCATAACGGCAAATGGAAATTACCACACGGATTGACCCCGACAACGCATATTTTCAAATTGCCTATGGG
AGAAATTCGTACCCCTAAACACCTGCTTGATCTGTCAAAAAGTGTAGATAACGAACTCGTCTGTTTGAGATTATTTTCTG
CTTTCAATATCCCTTCCGCAAACGTTGAATTATTGGAAATGAACGATATACGGGCATTAGCTGTTGAACGTTTTGATCGC
CGTCTTAGTCAAGATGGCAGTTATTTTTTACGTTTACCTTACGAAGATTTCTGTCAGGCATTCGGCTTATCGCCAACGCA
AAAATATGAAAGCGATGGTGGAATTGGTATTGGGGATATGATGAATAAGTTGCTCTACTCCAGTAATTCCAACGATCGCC
GTCAATTTATGCAAACTCAATTTCTGTTTTGGCTACTGGCTGCCATTGATGGACACGCTAAAAACTTTTCTCTCGCCATT
GAAAAACAAGGGCGTTATCACTTATCCCCAGTGTATGACGTACTTTCTGCTTATCCAATGTTAGGCGGAAAAGGTTGGCA
TCACAGTGAATTACAAATGGCAATGAGCTTGCGTAGCACGCAAAAAGGGAAAAAATGGCATTGGAGTAAAATTTATGCTG
AACATTTCTATGCCACAGCTAAGTTTGTCGGATTTAGCTCAAGTGATATGAAACAAGTGATAGAGCAAATTTGCGATACA
GTGCAAGAAACCATTGATAGCGTAGCAACAACACTACCCAAACAGATAGATGAAAATGTACGGGATCAAATATTCACGGG
CATAATGAAACAGGTGCAAAAAGTTTCTATGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9068.48 Da
• Isoelectric Point: 9.7687

>AT68590 WP_005713287.1 NZ_CP018034:c134418-134179 [Glaesserella parasuis str. Nagasaki]
MLITTPDMLGNSLRDIRKQKKLSQMQIANQVALRQATISTFEKKTDKATIETLFKLLSGLGYELHIHPRPEKTEGNDEW

Nucleotide

• Length: 240 bp

>AT68590 NZ_CP018034:c134418-134179 [Glaesserella parasuis str. Nagasaki]
ATGTTAATCACGACACCAGATATGCTTGGAAATAGCCTACGCGATATTCGTAAGCAAAAAAAGCTTTCTCAAATGCAAAT
AGCAAATCAAGTAGCTTTAAGACAAGCCACGATTTCCACCTTTGAGAAAAAAACCGATAAAGCCACTATTGAAACATTAT
TTAAGTTACTTTCAGGTTTAGGCTATGAACTACATATCCACCCTCGTCCTGAAAAAACAGAGGGGAATGATGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A836YYU2