Detailed information of TA system TA068588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1824211..1824988
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: U4SQ43
• Locus tag: A2U20_RS08720
• Protein ID: WP_021116693.1
• Coordinates: 1824211..1824711 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: A2U20_RS08725
• Protein ID: WP_005710653.1
• Coordinates: 1824701..1824988 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS08685	1819337..1819492	-	156	WP_021111605.1	YoaH family protein	-
A2U20_RS08690	1819510..1820238	-	729	WP_021111606.1	tRNA pseudouridine(65) synthase TruC	-
A2U20_RS08695	1820235..1820549	-	315	WP_005710664.1	YqcC family protein	-
A2U20_RS08700	1820591..1821505	-	915	WP_021116690.1	N-acetylmuramic acid 6-phosphate etherase	-
A2U20_RS08705	1821517..1822632	-	1116	WP_043895645.1	anhydro-N-acetylmuramic acid kinase	-
A2U20_RS08710	1822786..1823121	-	336	WP_016527793.1	outer membrane protein assembly factor BamE	-
A2U20_RS08715	1823182..1824183	-	1002	WP_021116692.1	nucleoid-associated protein YejK	-
A2U20_RS08720	1824211..1824711	-	501	WP_021116693.1	hypothetical protein	Toxin
A2U20_RS08725	1824701..1824988	-	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
A2U20_RS08730	1825134..1825523	+	390	WP_021116694.1	RidA family protein	-
A2U20_RS08735	1825527..1826264	+	738	WP_021116695.1	peptidoglycan editing factor PgeF	-
A2U20_RS08740	1826439..1828016	+	1578	WP_021116696.1	FAD-binding protein	-
A2U20_RS08745	1828121..1828621	+	501	WP_021115009.1	CvpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19113.26 Da
• Isoelectric Point: 9.0168

>T68588 WP_021116693.1 NZ_CP018032:c1824711-1824211 [Glaesserella parasuis D74]
MKSKFIEEPLTKHHQREAFDCGNLEMNRFFKQYARQSHEKGTSKTYISRHLDSQQIIGFYTITLSALDQQHLPELCKKRF
GYHPIPLFTLARLAVDIRYQKQDIGGMLLVKALKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMPLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T68588 NZ_CP018032:c1824711-1824211 [Glaesserella parasuis D74]
ATGAAGAGTAAGTTTATTGAAGAGCCTTTAACGAAGCATCATCAACGAGAAGCCTTTGATTGCGGAAATCTAGAGATGAA
CCGATTTTTTAAACAATACGCTCGGCAATCTCACGAAAAAGGGACATCAAAAACTTACATTAGCCGACACTTAGACAGTC
AACAGATTATCGGATTTTATACAATCACCTTAAGTGCATTAGACCAGCAACACTTACCCGAACTATGCAAAAAACGTTTT
GGCTACCACCCTATTCCGCTCTTTACATTAGCAAGACTTGCAGTGGATATTCGCTATCAAAAACAAGACATTGGCGGAAT
GCTGTTGGTTAAAGCTCTCAAACGCTGTGCCATCGTTGCCGAACAAGTCGGGGGAATTGGGCTTCTCATTGAAGCAAAAG
ATGAAGACATTTCTCGTTGGTATCAATCTTATGGTGCTATTCCATTGGAAAATATGCCATTAACCTTAATTCTACTCTTT
GATACAATCAAAGGACTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT68588 WP_005710653.1 NZ_CP018032:c1824988-1824701 [Glaesserella parasuis D74]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT68588 NZ_CP018032:c1824988-1824701 [Glaesserella parasuis D74]
ATGCCACAAATTGCAAATGCCCTTAACGATAGAATGTCTATTCGCATTAGTACGGAAGACAAGCAGACTTTAATTAGAGC
CGTTGCACTCACCCATACGAATATTACAGAATTTGTATTACAACGCATTTTGCCTAGTGCCAGAGAAATTATTCAGCAGT
ATGATAATCGGCAATTTTCACCGCAAGATCTCTCGTTGATTATGGAATTGCTTGATAATCCGCCGAAAGCTAATACAAAA
CTACAACAAGCGGCGAAATTAGCAAAGGAAAGCTATCATGAAGAGTAA

Structures

Toxin

• AlphaFold DB: U4SQ43

Antitoxin

• AlphaFold DB: U4SPD7