Detailed information of TA system TA068586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1191900..1192513
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A2U20_RS05775
• Protein ID: WP_200859958.1
• Coordinates: 1191900..1192067 (+)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U4SMI8
• Locus tag: A2U20_RS05780
• Protein ID: WP_021110735.1
• Coordinates: 1192106..1192513 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS05735	1187084..1187278	-	195	WP_021110729.1	hypothetical protein	-
A2U20_RS05740	1187478..1188302	-	825	WP_021116916.1	phage antirepressor N-terminal domain-containing protein	-
A2U20_RS12135	1188617..1188784	-	168	WP_021110921.1	hypothetical protein	-
A2U20_RS05745	1189081..1189299	+	219	WP_021116917.1	hypothetical protein	-
A2U20_RS05750	1189352..1190170	-	819	WP_043895698.1	damage-inducible protein D	-
A2U20_RS05755	1190535..1190720	+	186	WP_021116919.1	hypothetical protein	-
A2U20_RS05760	1190729..1191106	-	378	WP_021116920.1	hypothetical protein	-
A2U20_RS05765	1191103..1191507	-	405	WP_021116921.1	RusA family crossover junction endodeoxyribonuclease	-
A2U20_RS05770	1191504..1191794	-	291	WP_021110734.1	DUF1364 domain-containing protein	-
A2U20_RS05775	1191900..1192067	+	168	WP_200859958.1	type II toxin-antitoxin system HicA family toxin	Toxin
A2U20_RS05780	1192106..1192513	+	408	WP_021110735.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A2U20_RS05785	1192547..1192975	-	429	WP_021116922.1	recombination protein NinB	-
A2U20_RS05790	1192978..1193652	-	675	WP_021116923.1	replication P family protein	-
A2U20_RS05795	1193652..1194437	-	786	WP_021116924.1	replication protein	-
A2U20_RS05800	1194434..1195018	-	585	WP_021116925.1	phage antirepressor KilAC domain-containing protein	-
A2U20_RS05805	1195063..1195338	-	276	WP_021116926.1	hypothetical protein	-
A2U20_RS05810	1195359..1195565	-	207	WP_012621794.1	helix-turn-helix transcriptional regulator	-
A2U20_RS05815	1195700..1196353	+	654	WP_005714960.1	LexA family transcriptional regulator	-
A2U20_RS05820	1196415..1197266	+	852	WP_042905671.1	KilA-N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1179090..1216786	37696

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-55)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6272.42 Da
• Isoelectric Point: 11.0319

>T68586 WP_200859958.1 NZ_CP018032:1191900-1192067 [Glaesserella parasuis D74]
MIKLLKKNGWELDSIVGSHHHFEKKNQKGAGKVTVPHPRKDLGFLEKKIRKQAGL

Nucleotide

• Length: 168 bp

>T68586 NZ_CP018032:1191900-1192067 [Glaesserella parasuis D74]
ATGATAAAACTTCTCAAAAAGAACGGTTGGGAGCTAGATAGCATAGTCGGTAGCCACCACCACTTTGAGAAGAAAAATCA
AAAGGGAGCAGGCAAAGTGACTGTTCCCCACCCGAGAAAAGACTTAGGCTTTCTCGAAAAGAAAATCAGAAAACAAGCGG
GGCTGTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14904.94 Da
• Isoelectric Point: 4.6143

>AT68586 WP_021110735.1 NZ_CP018032:1192106-1192513 [Glaesserella parasuis D74]
MLYPLIFEQVSDGYVVAIPDIDGCYSAGDTLEETYSNVKQAIALHLESVVKDGGEIPTPTAIENHKNDPDLDGHNVFFGV
VDVDLSHLMGKAEKINITLPSYLIKRIDDFVAVHKEYKNRSNFLAKIAADKILTA

Nucleotide

• Length: 408 bp

>AT68586 NZ_CP018032:1192106-1192513 [Glaesserella parasuis D74]
ATGTTATACCCACTCATTTTTGAACAAGTTTCCGACGGCTATGTCGTTGCTATCCCAGATATTGACGGCTGTTATTCTGC
TGGAGATACCCTAGAAGAAACTTATAGCAACGTAAAACAAGCTATAGCACTTCATCTCGAATCTGTCGTCAAAGATGGGG
GAGAAATTCCAACGCCTACCGCTATCGAAAACCACAAAAACGACCCTGATTTAGACGGTCATAACGTATTTTTCGGTGTG
GTTGATGTCGATTTATCCCACCTAATGGGCAAAGCAGAGAAAATCAACATCACCCTCCCTAGCTATCTCATCAAACGCAT
TGATGATTTTGTTGCGGTTCACAAAGAGTATAAAAACCGTAGCAACTTCTTAGCTAAAATCGCAGCGGATAAAATCTTAA
CCGCTTAA

Structures

Antitoxin

• AlphaFold DB: U4SMI8