Detailed information of TA system TA068584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 632680..633260
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U4SBB4
• Locus tag: A2U20_RS03285
• Protein ID: WP_021113409.1
• Coordinates: 632680..633024 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U4SAI4
• Locus tag: A2U20_RS03290
• Protein ID: WP_005710601.1
• Coordinates: 633021..633260 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS03275	630602..631993	-	1392	WP_021117914.1	MATE family efflux transporter	-
A2U20_RS03280	632006..632674	-	669	WP_021113308.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
A2U20_RS03285	632680..633024	-	345	WP_021113409.1	endoribonuclease MazF	Toxin
A2U20_RS03290	633021..633260	-	240	WP_005710601.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A2U20_RS03295	633446..634321	+	876	WP_021113313.1	putative selenate ABC transporter substrate-binding protein	-
A2U20_RS03300	634432..635181	+	750	WP_021117915.1	phosphonate ABC transporter ATP-binding protein	-
A2U20_RS03305	635171..635956	+	786	WP_021112027.1	ABC transporter permease subunit	-
A2U20_RS03310	635953..636801	+	849	WP_021112028.1	ABC transporter permease subunit	-
A2U20_RS03315	636872..637564	+	693	WP_021117916.1	tellurite resistance TerB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12800.79 Da
• Isoelectric Point: 7.9277

>T68584 WP_021113409.1 NZ_CP018032:c633024-632680 [Glaesserella parasuis D74]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPAIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Nucleotide

• Length: 345 bp

>T68584 NZ_CP018032:c633024-632680 [Glaesserella parasuis D74]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGTCATCGTCCAGCATTAGTGTTAACGCCCGCGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCACTCACAA
CAAAAGTAAAAGGCTATCCTTTCGAAGTCAATATTGCAGGTACCCCCCAAAATGTTGTGCTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8782.21 Da
• Isoelectric Point: 5.0351

>AT68584 WP_005710601.1 NZ_CP018032:c633260-633021 [Glaesserella parasuis D74]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Nucleotide

• Length: 240 bp

>AT68584 NZ_CP018032:c633260-633021 [Glaesserella parasuis D74]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCAGGTATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Structures

Toxin

• AlphaFold DB: U4SBB4

Antitoxin

• AlphaFold DB: U4SAI4