Detailed information of TA system TA068582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 395307..396994
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U4SB19
• Locus tag: A2U20_RS02135
• Protein ID: WP_021111224.1
• Coordinates: 395307..396650 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: A2U20_RS02140
• Protein ID: WP_015940035.1
• Coordinates: 396650..396994 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS02110	391277..392119	-	843	WP_021111219.1	TonB-dependent receptor	-
A2U20_RS02115	392129..392353	+	225	Protein_400	hypothetical protein	-
A2U20_RS02120	392596..394275	+	1680	WP_005713398.1	DNA repair protein RecN	-
A2U20_RS02125	394355..394582	-	228	Protein_402	transposase	-
A2U20_RS02130	394756..395193	+	438	WP_021117780.1	ribosomal protein S18-alanine N-acetyltransferase	-
A2U20_RS02135	395307..396650	+	1344	WP_021111224.1	type II toxin-antitoxin system HipA family toxin	Toxin
A2U20_RS02140	396650..396994	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
A2U20_RS02145	397023..398348	+	1326	WP_021117781.1	ATP-dependent RNA helicase SrmB	-
A2U20_RS02150	398469..399101	+	633	WP_021110345.1	lipoprotein insertase outer membrane protein LolB	-
A2U20_RS02155	399103..399948	+	846	WP_021113204.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
A2U20_RS02160	399976..400926	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	394355..394606	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51106.20 Da
• Isoelectric Point: 5.4867

>T68582 WP_021111224.1 NZ_CP018032:395307-396650 [Glaesserella parasuis D74]
MQIEPLILQAYIADRWQDIAILTFQADYSLNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIETSHLVETGMFFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T68582 NZ_CP018032:395307-396650 [Glaesserella parasuis D74]
ATGCAAATCGAACCGCTTATACTTCAAGCCTACATTGCTGATCGTTGGCAGGATATTGCTATTTTGACCTTTCAGGCTGA
TTATAGTCTCAATGATTTTAGCTATCTCCATGATTTAACAGAAATTTTAAGTGATAATGATAAGGCAGTTTCATTTAACT
ACCCTGTGGAATTTTTTCCGCATCGCAGTGTATCGGGCTGGTTTGCATTTTTAGATGATATAGTCCCTTCTGGGGCAAGC
CGGCGTTATTGGTTGCAAAAACTAGATATTGCTCATTTGCCGATGGCTCAACAAAACTATTTGTTATTAAAACAAGGCAC
GATTGCACCTGTCGGGCATTTACGAGTTAAACAAGCGGTCAGTTCTTTTGTCGAAAATGAACCCTCTCGCTTATTTGAAA
TAGACGATGTTATCAATCGCCAAGCAGACTTTTTGGAATACGCTAATCAGCGTGGGGCAATGGCTGGCGGTGCCACAGGG
GCTGGCGGTGAAGCACCGAAATTATTGCTACGCCAAACAGCCACTCGGCAAGTTTGGATTGATAACCTACAACAAGGTGC
CTCTTCTGACGATTTCTATTTAGTCAAGTTTCCGCGTGGGAAACGTAGTGTAATAGATTGCGATATATTGAGAGCTGAAT
TTCACTATTATCACGAATTAGCCTCAATGGGTTTTGACACGATTGATACGACAAAAATGCGATTGGAAGAAGGCGAACGT
TACCCGTCGTTGTGGTTACCTCGTTTTGATGTTTATCGTAATGAACAGGGGAAAATGGCTCGTTATGCCGTTGAATCTGT
TTATGCTATGTTAGAAAAAGGGGCTGGCTCTTTGCTGGATCACGAAAAGGTTATTCGTCAGTTGATTGCTAAAATTGAAA
CCTCTCATTTGGTTGAAACAGGAATGTTTTTTGATAAACAAGCTTTTGTGATCGAGTGGGTAAGGCGAGATTTATTGAAT
ATCGCATTTGGAAATAGCGATAATCACGGGCGAAATACGGCTTTCTTACGCAATCAAAATCGTATCTGGCTTGCTCCAAT
TTATGATTTTGCCCCAATGAAAGCAGATCCTGAAGGTATTCCTCGCACGACAATGTGGGATAGTAAAGGTCACCCTGCAT
TAGAATTAGGCGGTGAATATCGTTTTGATCTGATTGCAGAACAGCTTGATGATTTATGTCCAGCAGACAAGTTATTGGAT
GCTTTGCGTCAAACAGCTCAACAGCTTGTCGGGCTTAAAGCACGTTTAGAAGCAAGGGGCGTTCCAACGGCGATTTTGAC
GTACCCTTCAATCAGTTTGGACTATTTACCTGAAAAATTAAAACGTTGGCAATTATTATCATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT68582 WP_015940035.1 NZ_CP018032:396650-396994 [Glaesserella parasuis D74]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT68582 NZ_CP018032:396650-396994 [Glaesserella parasuis D74]
ATGGAAGAGATCAAACAGGCGAGAGCCTCTAAAAGTAAAAAAACGTTAGATATTTATCAGCGAGCGACTGTGCGAGACGA
AATTGCTCGTAAGCTATTGCTCAATGAGATGAGTTTAGGGCAGGCGTTGAAATATCTACGTTTACATCTACTGGCAATGA
AACAAGAGCGTTATGCGGAGATTGTCAAAGTTTCTCGCAAAACGTTATCTGATTTAGAAAATGATAAGGGAAATTACAGT
ATTGATATTATCAATCAGGTGTTACGCCCCTTTGAATTACAACTAGGTGTTGTGCCAATGAACAAAACACTACTCAGACA
AGTTTTAAACGAACAAGCGGTCTGA

Structures

Toxin

• AlphaFold DB: U4SB19