Detailed information of TA system TA068581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 265287..265926
• Replicon: chromosome
• Accession: NZ_CP018032
• Organism: Glaesserella parasuis D74

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A2U20_RS01515
• Protein ID: WP_148122863.1
• Coordinates: 265591..265926 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A2U20_RS01510
• Protein ID: WP_164681833.1
• Coordinates: 265287..265583 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2U20_RS01475	260381..261220	+	840	WP_021117455.1	hypothetical protein	-
A2U20_RS01480	261249..261665	+	417	WP_021117454.1	hypothetical protein	-
A2U20_RS01485	261679..261930	+	252	WP_042906724.1	hypothetical protein	-
A2U20_RS01490	262156..262446	+	291	WP_043895876.1	DUF805 domain-containing protein	-
A2U20_RS01495	262449..262862	+	414	WP_021117451.1	hypothetical protein	-
A2U20_RS01500	262997..264124	-	1128	Protein_287	IS3 family transposase	-
A2U20_RS01505	264225..265214	-	990	WP_021110908.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
A2U20_RS01510	265287..265583	-	297	WP_164681833.1	putative addiction module antidote protein	Antitoxin
A2U20_RS01515	265591..265926	-	336	WP_148122863.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A2U20_RS01520	266085..266489	+	405	WP_021113195.1	DNA polymerase III subunit psi	-
A2U20_RS01525	266604..266915	+	312	WP_042906459.1	hypothetical protein	-
A2U20_RS01530	267009..267293	+	285	WP_148122864.1	hypothetical protein	-
A2U20_RS01535	267369..267839	+	471	WP_021110902.1	hypothetical protein	-
A2U20_RS01540	268038..268781	+	744	Protein_295	transposase	-
A2U20_RS01545	268971..269156	-	186	WP_005713975.1	zf-HC2 domain-containing protein	-
A2U20_RS01550	269153..269725	-	573	WP_035522608.1	sigma-70 family RNA polymerase sigma factor	-
A2U20_RS01555	269866..270195	+	330	WP_021110897.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	262997..274350	11353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-93)

Antitoxin

• Uncharacterized protein HI1420 (9-91)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12107.82 Da
• Isoelectric Point: 9.8168

>T68581 WP_148122863.1 NZ_CP018032:c265926-265591 [Glaesserella parasuis D74]
MSGIINQIVITDNFSKWLDGLKNPIAKATVARRIKNAVKGNFGDHKALAGTGGLYEMRIATGAGYRIYYAQQGDVIYILL
QGGDKSTQQVDIEKAKALWAELKQTGGSNEY

Nucleotide

• Length: 336 bp

>T68581 NZ_CP018032:c265926-265591 [Glaesserella parasuis D74]
ATGTCAGGAATTATCAACCAAATCGTCATTACAGATAATTTTTCAAAGTGGCTAGATGGTTTAAAAAATCCTATTGCTAA
AGCAACAGTGGCAAGAAGAATTAAGAATGCAGTAAAAGGTAATTTTGGTGATCATAAAGCGCTTGCAGGTACTGGTGGAC
TTTATGAAATGCGAATAGCGACAGGCGCTGGTTATCGAATTTACTATGCACAGCAGGGCGATGTTATTTATATTCTGTTA
CAAGGTGGTGATAAATCAACACAGCAAGTCGATATTGAGAAAGCGAAAGCATTGTGGGCAGAATTAAAACAGACAGGAGG
AAGTAATGAATATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10948.49 Da
• Isoelectric Point: 4.8951

>AT68581 WP_164681833.1 NZ_CP018032:c265583-265287 [Glaesserella parasuis D74]
MAEKHGLRPFDVAEFLDNEETIQAYLAEVLKEGDQDEFLEALNDVARARGIAELAKQTGLGRESLYKTLSKGSKPRFETI
QKILGAFNLVLIPQIKNS

Nucleotide

• Length: 297 bp

>AT68581 NZ_CP018032:c265583-265287 [Glaesserella parasuis D74]
ATGGCAGAAAAACACGGTTTACGTCCTTTTGATGTCGCTGAATTTTTGGATAACGAAGAAACAATACAAGCCTATTTAGC
AGAAGTATTAAAAGAGGGCGATCAAGATGAGTTTTTGGAAGCCCTTAATGATGTAGCAAGAGCAAGGGGGATCGCAGAAC
TTGCGAAGCAAACGGGGTTAGGTAGAGAAAGTTTATATAAAACCCTATCTAAAGGAAGTAAACCTCGTTTTGAAACTATT
CAAAAAATTTTAGGAGCATTTAATCTTGTGCTTATCCCACAAATAAAAAATAGTTAA