Detailed information of TA system TA068550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 17729..18279
• Replicon: plasmid pKrDSM16656L
• Accession: NZ_CP018017
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: Y71_RS28130
• Protein ID: WP_007372286.1
• Coordinates: 17729..18037 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: Y71_RS28135
• Protein ID: WP_007372285.1
• Coordinates: 18040..18279 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS28090	12811..13254	-	444	WP_007372295.1	hypothetical protein	-
Y71_RS28095	13492..13830	+	339	WP_007372294.1	hypothetical protein	-
Y71_RS28100	14193..14519	-	327	WP_007372293.1	hypothetical protein	-
Y71_RS28105	14603..15001	-	399	WP_007372292.1	hypothetical protein	-
Y71_RS28110	15012..15254	-	243	WP_007372291.1	hypothetical protein	-
Y71_RS28115	15601..16071	-	471	WP_007372290.1	hypothetical protein	-
Y71_RS28120	16154..16771	-	618	WP_007372289.1	hypothetical protein	-
Y71_RS28125	16841..17395	-	555	WP_007372288.1	hypothetical protein	-
Y71_RS30460	17550..17708	-	159	WP_016241558.1	hypothetical protein	-
Y71_RS28130	17729..18037	-	309	WP_007372286.1	CcdB family protein	Toxin
Y71_RS28135	18040..18279	-	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
Y71_RS30545	18418..18882	-	465	WP_008786596.1	hypothetical protein	-
Y71_RS28145	18879..19550	-	672	WP_007372283.1	hypothetical protein	-
Y71_RS28150	20011..20643	-	633	WP_007372282.1	hypothetical protein	-
Y71_RS30465	20659..20799	-	141	WP_007372281.1	hypothetical protein	-
Y71_RS28155	20947..21405	-	459	WP_035942286.1	hypothetical protein	-
Y71_RS28160	21458..21655	-	198	WP_032155220.1	Lar family restriction alleviation protein	-
Y71_RS28165	21678..22373	-	696	WP_007372278.1	hypothetical protein	-
Y71_RS28170	22504..22695	-	192	WP_016241560.1	hypothetical protein	-
Y71_RS28175	22877..23272	-	396	WP_024196081.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..290108	290108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T68550 WP_007372286.1 NZ_CP018017:c18037-17729 [Kosakonia radicincitans DSM 16656]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T68550 NZ_CP018017:c18037-17729 [Kosakonia radicincitans DSM 16656]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT68550 WP_007372285.1 NZ_CP018017:c18279-18040 [Kosakonia radicincitans DSM 16656]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT68550 NZ_CP018017:c18279-18040 [Kosakonia radicincitans DSM 16656]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3