Detailed information of TA system TA068549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 10079..10980
• Replicon: plasmid pKrDSM16656L
• Accession: NZ_CP018017
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: Y71_RS28065
• Protein ID: WP_007372300.1
• Coordinates: 10522..10980 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: Y71_RS28060
• Protein ID: WP_008786588.1
• Coordinates: 10079..10525 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS28030	5442..5693	-	252	WP_024196080.1	hypothetical protein	-
Y71_RS28035	6115..6957	-	843	WP_007372307.1	HNH endonuclease	-
Y71_RS30455	7525..7689	-	165	WP_007372306.1	hypothetical protein	-
Y71_RS28040	7976..8419	-	444	WP_007372305.1	hypothetical protein	-
Y71_RS28045	8479..8757	-	279	WP_007372304.1	hypothetical protein	-
Y71_RS28050	8729..9571	-	843	WP_007372303.1	hypothetical protein	-
Y71_RS28055	9613..9930	-	318	WP_007372302.1	hypothetical protein	-
Y71_RS28060	10079..10525	+	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
Y71_RS28065	10522..10980	+	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
Y71_RS28070	11024..11254	+	231	WP_007372299.1	hypothetical protein	-
Y71_RS28075	11322..11588	-	267	WP_007372298.1	hypothetical protein	-
Y71_RS28080	11638..12192	-	555	WP_007372297.1	hypothetical protein	-
Y71_RS28085	12351..12692	-	342	WP_007372296.1	hypothetical protein	-
Y71_RS28090	12811..13254	-	444	WP_007372295.1	hypothetical protein	-
Y71_RS28095	13492..13830	+	339	WP_007372294.1	hypothetical protein	-
Y71_RS28100	14193..14519	-	327	WP_007372293.1	hypothetical protein	-
Y71_RS28105	14603..15001	-	399	WP_007372292.1	hypothetical protein	-
Y71_RS28110	15012..15254	-	243	WP_007372291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..290108	290108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T68549 WP_007372300.1 NZ_CP018017:10522-10980 [Kosakonia radicincitans DSM 16656]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T68549 NZ_CP018017:10522-10980 [Kosakonia radicincitans DSM 16656]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT68549 WP_008786588.1 NZ_CP018017:10079-10525 [Kosakonia radicincitans DSM 16656]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT68549 NZ_CP018017:10079-10525 [Kosakonia radicincitans DSM 16656]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8