Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5283441..5284198 Replicon chromosome
Accession NZ_CP018016
Organism Kosakonia radicincitans DSM 16656

Toxin (Protein)


Gene name tacT Uniprot ID A0A7H0RWY0
Locus tag Y71_RS25435 Protein ID WP_007372585.1
Coordinates 5283441..5283923 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag Y71_RS25440 Protein ID WP_007372584.1
Coordinates 5283914..5284198 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y71_RS25415 5278501..5279781 - 1281 WP_071532028.1 type 4b pilus protein PilO2 -
Y71_RS25420 5279784..5281457 - 1674 WP_007372588.1 PilN family type IVB pilus formation outer membrane protein -
Y71_RS25425 5281477..5281920 - 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
Y71_RS25430 5281922..5283208 - 1287 WP_007372586.1 TcpQ domain-containing protein -
Y71_RS25435 5283441..5283923 - 483 WP_007372585.1 GNAT family N-acetyltransferase Toxin
Y71_RS25440 5283914..5284198 - 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
Y71_RS25445 5284354..5284854 - 501 WP_007372583.1 single-stranded DNA-binding protein -
Y71_RS25450 5284930..5285421 - 492 WP_007372582.1 DUF3577 domain-containing protein -
Y71_RS25455 5286054..5288066 - 2013 WP_007372580.1 DNA topoisomerase III -
Y71_RS25460 5288084..5288806 - 723 WP_007372579.1 TIGR03761 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5189248..5296422 107174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17756.41 Da        Isoelectric Point: 7.3823

>T68547 WP_007372585.1 NZ_CP018016:c5283923-5283441 [Kosakonia radicincitans DSM 16656]
MEINVTAPALLTEEHVLHPFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTARVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T68547 NZ_CP018016:c5283923-5283441 [Kosakonia radicincitans DSM 16656]
ATGGAAATAAATGTCACTGCGCCTGCTTTACTGACAGAGGAGCATGTTCTTCATCCGTTCGATTGCGGGAATGCGGTACT
TGATGACTGGCTTCGCCGGCGTGCGTTGAAGAATCAGCATCTTAATGCTTCCCGTACATTCGTCATCTGTCCTGAAGGTA
CTGCGCGTGTAGTGGGTTATTATTCCCTCGCGACTGGCTCAGTAAGTCATGTCGACTTGAGTCGTAGTCTGCGACAGAAT
ATGCCCGATCCCGTACCGGTTGTTTTGCTGGGCCGCCTTGCAGTCGATGTATCCACTCAGGGCAATAACTTCGGGAAATG
GTTGCTGAATGATGCTGTGATGCGCGTATCGAATCTGGCGGATCAGGTCGGCATCAAGGCTATCATGGTGCATGCAATTG
ATGAGCGGGCTAAAGCGTTCTATGAATACTTTGGTTTTGTGCAGTCACCAGTTGCGGCTAACACGCTTTTCTACAAAATA
TGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT68547 WP_007372584.1 NZ_CP018016:c5284198-5283914 [Kosakonia radicincitans DSM 16656]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT68547 NZ_CP018016:c5284198-5283914 [Kosakonia radicincitans DSM 16656]
ATGCAAACTCCAGTCCGTAAATCCGTCCGTGACAAGCAAATCAATATCCGCGCCACGGATGAAGAACGTGCGGTGATTGA
TTACGCTGCGAGTCTGGTTAATAAAAACCGTACTGATTTCATCATTGAAAGGGCTGTGCATGAGGCCCAGAACATCATTC
TGGACCAGCGTGTGTTCGTTCTTGATGATGCCCGTTACCAGGCGTTCATCAGGCAGCTTGAAGCCCCTGTGCAGAATGTT
GAAGGTCGTCAGCGTCTGATGGATGTAAAACCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0RWY0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References