Detailed information of TA system TA068545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4873313..4873767
• Replicon: chromosome
• Accession: NZ_CP018016
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: Y71_RS23405
• Protein ID: WP_007373008.1
• Coordinates: 4873633..4873767 (-)
• Length: 45 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: Y71_RS23400
• Protein ID: WP_007373009.1
• Coordinates: 4873313..4873618 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS23385	4869761..4871914	+	2154	WP_007373012.1	pyruvate/proton symporter BtsT	-
Y71_RS23390	4872065..4872268	+	204	WP_007373011.1	YbdD/YjiX family protein	-
Y71_RS23395	4872279..4873235	+	957	WP_007373010.1	GTPase	-
Y71_RS23400	4873313..4873618	-	306	WP_007373009.1	BrnA antitoxin family protein	Antitoxin
Y71_RS23405	4873633..4873767	-	135	WP_007373008.1	BrnT family toxin	Toxin
Y71_RS23410	4873894..4875264	-	1371	WP_007373007.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
Y71_RS23415	4875463..4875984	+	522	WP_007373006.1	VOC family protein	-
Y71_RS23420	4875986..4877089	-	1104	WP_007373005.1	M20 peptidase aminoacylase family protein	-
Y71_RS23425	4877114..4877581	-	468	WP_007373004.1	YjiG family protein	-
Y71_RS23430	4877574..4878257	-	684	WP_007373003.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-41)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-93)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 5328.82 Da
• Isoelectric Point: 4.6615

>T68545 WP_007373008.1 NZ_CP018016:c4873767-4873633 [Kosakonia radicincitans DSM 16656]
MEFEWDVTKARSNQRKHGIRFEEAVSVFEDPYHLSIQDRFENGE

Nucleotide

• Length: 135 bp

>T68545 NZ_CP018016:c4873767-4873633 [Kosakonia radicincitans DSM 16656]
ATGGAGTTTGAATGGGACGTCACTAAAGCAAGAAGTAATCAACGCAAGCATGGCATTCGCTTTGAAGAAGCCGTATCGGT
TTTCGAGGATCCTTACCATTTGTCCATCCAGGACCGGTTCGAGAATGGCGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11422.20 Da
• Isoelectric Point: 9.9057

>AT68545 WP_007373009.1 NZ_CP018016:c4873618-4873313 [Kosakonia radicincitans DSM 16656]
MSMVKHKSGELPALNKQCEAELKALANKSDGEIDYSDIPSTDDVLWAGAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMMRDLQNKK

Nucleotide

• Length: 306 bp

>AT68545 NZ_CP018016:c4873618-4873313 [Kosakonia radicincitans DSM 16656]
ATGAGCATGGTTAAACATAAATCGGGTGAGTTGCCAGCGCTCAACAAGCAGTGTGAAGCTGAATTAAAAGCGCTGGCAAA
TAAATCGGACGGAGAGATTGATTACAGCGATATTCCGTCCACAGATGACGTGTTATGGGCTGGCGCTGTGCGCGGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCATCAGTGCGTATTGATGCTGATGTGATGGAGTGGTTAAAACGACCGGGGAAAGGG
TATCAGACGCGGCTCAATGCCATTTTGCGTGAGGCAATGATGCGCGATTTGCAGAACAAGAAGTAA