Detailed information of TA system TA068542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4048265..4049059
• Replicon: chromosome
• Accession: NZ_CP018016
• Organism: Kosakonia radicincitans DSM 16656

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Y71_RS19560
• Protein ID: WP_007373816.1
• Coordinates: 4048538..4049059 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: Y71_RS19555
• Protein ID: WP_007373817.1
• Coordinates: 4048265..4048534 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y71_RS19530	4044426..4045040	+	615	WP_007373822.1	methylthioribulose 1-phosphate dehydratase	-
Y71_RS19535	4045037..4045726	+	690	WP_007373821.1	acireductone synthase	-
Y71_RS19540	4045723..4046265	+	543	WP_007373820.1	acireductone dioxygenase	-
Y71_RS19545	4046400..4047227	-	828	WP_007373819.1	hypothetical protein	-
Y71_RS30430	4047326..4047499	-	174	WP_167287342.1	hypothetical protein	-
Y71_RS19550	4047742..4048059	+	318	WP_007373818.1	hypothetical protein	-
Y71_RS19555	4048265..4048534	+	270	WP_007373817.1	DUF1778 domain-containing protein	Antitoxin
Y71_RS19560	4048538..4049059	+	522	WP_007373816.1	GNAT family N-acetyltransferase	Toxin
Y71_RS19565	4049093..4049320	-	228	WP_035943538.1	helix-turn-helix domain-containing protein	-
Y71_RS19570	4049571..4050143	+	573	WP_007373814.1	DUF1349 domain-containing protein	-
Y71_RS19575	4050574..4052112	+	1539	WP_007373813.1	MCP four helix bundle domain-containing protein	-
Y71_RS19580	4052197..4052457	-	261	WP_035886084.1	MafI family immunity protein	-
Y71_RS19585	4052457..4052786	-	330	WP_007373811.1	SdpI family protein	-
Y71_RS19590	4053016..4053789	-	774	WP_007373810.1	hypothetical protein	-
Y71_RS19595	4053792..4053989	-	198	WP_007373809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19485.38 Da
• Isoelectric Point: 8.4708

>T68542 WP_007373816.1 NZ_CP018016:4048538-4049059 [Kosakonia radicincitans DSM 16656]
VDNLTIEMFSGETEYETGGFDCGEVSLNAFLTNHLKRQHDGKILRAWLLVTKTPRPRILGYYTLSGGSFEKEQLPTKTQQ
KKVPYHNVPCVILGRLAIDKSLQGNGWGETLVAHAMRVVYNASLTIGIHGLFVEALTGNAKKFYEKLGFIPLADRNQHSL
FFPTKSIERLFEE

Nucleotide

• Length: 522 bp

>T68542 NZ_CP018016:4048538-4049059 [Kosakonia radicincitans DSM 16656]
GTGGACAACCTGACCATTGAGATGTTCTCAGGAGAGACAGAGTATGAAACAGGAGGTTTTGATTGTGGTGAGGTATCGCT
CAACGCTTTTTTAACCAACCATCTCAAACGCCAGCATGATGGCAAAATTTTACGTGCCTGGCTCCTTGTAACGAAAACGC
CCAGGCCGCGCATTTTAGGCTATTACACCTTGTCTGGCGGCAGTTTCGAGAAAGAACAACTGCCCACGAAAACACAGCAA
AAAAAGGTGCCTTATCACAATGTCCCCTGCGTCATATTAGGTCGGCTCGCTATTGATAAGTCACTCCAGGGAAACGGCTG
GGGAGAAACCCTGGTGGCGCACGCCATGAGGGTGGTTTACAACGCTTCACTTACCATCGGCATACATGGGTTATTTGTCG
AAGCACTCACTGGCAACGCCAAAAAATTCTATGAAAAGCTGGGCTTCATTCCTTTAGCCGATCGCAATCAGCATTCGCTG
TTCTTTCCAACAAAGTCTATTGAACGCTTGTTCGAAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10273.77 Da
• Isoelectric Point: 7.0179

>AT68542 WP_007373817.1 NZ_CP018016:4048265-4048534 [Kosakonia radicincitans DSM 16656]
MPALKKQRIDLRLTDEDKKMIEEAAAMTNQTVTQFLVNSASERAAEVIEQHRRLILSEESWNRVMDAIENPPAPNDKLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT68542 NZ_CP018016:4048265-4048534 [Kosakonia radicincitans DSM 16656]
ATGCCCGCACTGAAAAAACAGCGCATAGATCTGCGACTAACGGATGAGGATAAGAAAATGATCGAAGAAGCGGCGGCAAT
GACCAATCAGACGGTCACACAGTTTCTGGTTAACAGCGCTTCTGAACGCGCCGCGGAAGTGATTGAACAGCACCGTCGCC
TGATTCTCAGCGAGGAATCATGGAACAGGGTTATGGATGCTATCGAAAACCCACCAGCACCGAATGACAAACTTAAACGC
GCCGCAAAACGTCTGCAAAACATGGAGTGA