Detailed information of TA system TA068524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3360706..3361343
• Replicon: chromosome
• Accession: NZ_CP018007
• Organism: Bacillus velezensis strain sx01604

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BLL65_RS16520
• Protein ID: WP_003156187.1
• Coordinates: 3360706..3361056 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BLL65_RS16525
• Protein ID: WP_003156188.1
• Coordinates: 3361062..3361343 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLL65_RS16480	3355746..3356348	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
BLL65_RS16485	3356348..3357136	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
BLL65_RS16490	3357102..3357584	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
BLL65_RS16495	3357581..3357910	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BLL65_RS16500	3357974..3358981	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BLL65_RS16505	3358993..3359394	-	402	WP_003156178.1	anti-sigma regulatory factor	-
BLL65_RS16510	3359397..3359762	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BLL65_RS16515	3359767..3360588	-	822	WP_003156182.1	STAS domain-containing protein	-
BLL65_RS16520	3360706..3361056	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BLL65_RS16525	3361062..3361343	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BLL65_RS16530	3361463..3362632	-	1170	WP_032873001.1	alanine racemase	-
BLL65_RS16535	3362749..3363756	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
BLL65_RS16540	3363921..3364286	-	366	WP_007609580.1	holo-ACP synthase	-
BLL65_RS16545	3364379..3364978	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68524 WP_003156187.1 NZ_CP018007:c3361056-3360706 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68524 NZ_CP018007:c3361056-3360706 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68524 WP_003156188.1 NZ_CP018007:c3361343-3361062 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68524 NZ_CP018007:c3361343-3361062 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5