Detailed information of TA system TA068522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 1874030..1874559
• Replicon: chromosome
• Accession: NZ_CP018005
• Organism: Coxiella burnetii strain RSA439

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: BMW92_RS10300
• Protein ID: WP_005769718.1
• Coordinates: 1874284..1874559 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: BMW92_RS10295
• Protein ID: WP_005769716.1
• Coordinates: 1874030..1874284 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMW92_RS10255	1869098..1869472	+	375	WP_005769706.1	hypothetical protein	-
BMW92_RS10260	1869517..1870215	+	699	WP_010958581.1	outer membrane beta-barrel protein	-
BMW92_RS10265	1870279..1871220	+	942	WP_005769709.1	S49 family peptidase	-
BMW92_RS10270	1871233..1872108	+	876	WP_010958582.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
BMW92_RS11335	1872084..1872197	+	114	Protein_1917	type II toxin-antitoxin system YoeB family toxin	-
BMW92_RS10280	1872257..1872391	+	135	WP_010958583.1	hypothetical protein	-
BMW92_RS10285	1872552..1873571	+	1020	WP_041952483.1	IS110-like element IS1111A family transposase	-
BMW92_RS10290	1873568..1873840	-	273	WP_010957319.1	hypothetical protein	-
BMW92_RS10295	1874030..1874284	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BMW92_RS10300	1874284..1874559	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
BMW92_RS10305	1874532..1875017	-	486	WP_005769725.1	dihydrofolate reductase	-
BMW92_RS10310	1875014..1875817	-	804	WP_010958584.1	epoxyqueuosine reductase QueH	-
BMW92_RS10315	1876013..1876300	+	288	WP_010958585.1	acylphosphatase	-
BMW92_RS10320	1876276..1876830	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
BMW92_RS10325	1876843..1877787	-	945	WP_010958586.1	methionyl-tRNA formyltransferase	-
BMW92_RS10330	1877975..1878901	+	927	WP_010958587.1	DNA-processing protein DprA	-
BMW92_RS10335	1878898..1879377	+	480	WP_010958588.1	DUF494 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1872552..1873571	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T68522 WP_005769718.1 NZ_CP018005:1874284-1874559 [Coxiella burnetii]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T68522 NZ_CP018005:1874284-1874559 [Coxiella burnetii]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT68522 WP_005769716.1 NZ_CP018005:1874030-1874284 [Coxiella burnetii]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT68522 NZ_CP018005:1874030-1874284 [Coxiella burnetii]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4