Detailed information of TA system TA068519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 88522..89312
• Replicon: plasmid pY9
• Accession: NZ_CP018001
• Organism: Rhizobium sp. Y9

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: BLX90_RS23835
• Protein ID: WP_082055484.1
• Coordinates: 88815..89312 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7W4U0T2
• Locus tag: BLX90_RS23830
• Protein ID: WP_044460092.1
• Coordinates: 88522..88818 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLX90_RS23805	84422..85135	+	714	WP_044460089.1	GntR family transcriptional regulator	-
BLX90_RS23810	85132..86052	+	921	WP_044460090.1	dihydrodipicolinate synthase family protein	-
BLX90_RS23815	86066..87205	+	1140	WP_044460091.1	mandelate racemase/muconate lactonizing enzyme family protein	-
BLX90_RS23825	87998..88129	-	132	Protein_95	LacI family transcriptional regulator	-
BLX90_RS23830	88522..88818	+	297	WP_044460092.1	DUF1778 domain-containing protein	Antitoxin
BLX90_RS23835	88815..89312	+	498	WP_082055484.1	GNAT family N-acetyltransferase	Toxin
BLX90_RS23840	89959..90469	+	511	Protein_98	glutathione S-transferase family protein	-
BLX90_RS23845	90557..91153	-	597	WP_044460093.1	gamma carbonic anhydrase family protein	-
BLX90_RS23850	91185..91565	-	381	WP_100911655.1	nuclear transport factor 2 family protein	-
BLX90_RS23855	91658..92692	+	1035	WP_044460095.1	transcriptional regulator FtrA	-
BLX90_RS23860	92726..93985	-	1260	WP_082055485.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB	1..481964	481964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17625.30 Da
• Isoelectric Point: 7.2441

>T68519 WP_082055484.1 NZ_CP018001:88815-89312 [Rhizobium sp. Y9]
VTLTSPAPLAEHHELAEFNSGVPELDDWLRRRARANQAGGASRTFVVCEGNRVVAYYALASGAVRTPEAPGRFKRNMPDP
IPIAVLGRLAIDLSYQGCGIGRALVRDAGLRLLNAAEILGIRGVLVHAISDDARAFYEAVGFMPSPSDPMMLMIGLKDLS
NALSA

Nucleotide

• Length: 498 bp

>T68519 NZ_CP018001:88815-89312 [Rhizobium sp. Y9]
GTGACCCTTACTTCCCCGGCTCCATTGGCTGAACATCATGAGCTTGCAGAGTTTAATTCTGGGGTCCCGGAGCTTGATGA
TTGGCTGCGTCGAAGAGCGCGAGCCAACCAGGCGGGCGGTGCATCACGTACTTTCGTTGTCTGTGAGGGGAACCGCGTCG
TTGCTTACTACGCTCTCGCCTCCGGAGCGGTCAGGACACCAGAAGCACCAGGTCGCTTCAAGCGAAACATGCCAGATCCG
ATCCCAATTGCTGTGCTTGGTCGACTCGCTATCGACCTTTCTTATCAAGGCTGCGGTATCGGACGGGCGTTGGTGCGTGA
TGCCGGCCTGCGTCTGCTGAACGCCGCTGAAATCCTCGGTATCCGCGGCGTACTGGTGCACGCGATATCCGATGACGCAC
GTGCATTCTATGAAGCTGTCGGATTCATGCCCTCTCCGTCCGATCCGATGATGTTGATGATCGGATTAAAAGATCTCAGC
AACGCACTGAGCGCGTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11100.69 Da
• Isoelectric Point: 9.6895

>AT68519 WP_044460092.1 NZ_CP018001:88522-88818 [Rhizobium sp. Y9]
MPTSHTSKNLDASVPLNIRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEAYAEYLTRLDAPAQ
SNERLKRTMSIKAPWDKA

Nucleotide

• Length: 297 bp

>AT68519 NZ_CP018001:88522-88818 [Rhizobium sp. Y9]
ATGCCGACGTCACACACGAGCAAAAACTTGGATGCAAGTGTTCCGCTGAATATCCGTATAAAACCCGCGACGCGCAATCT
CATCGATCGCGCGGCTGAGCTGCTTGGCAAGACACGGACGGATTTCATGCTGGAGGCCTCCGAACGCCGCGCCGAGGAAG
TGCTTCTTGACCGTACTGTATTCACGGTCAGCCCGGAGGCGTATGCTGAATACCTCACCCGGCTCGATGCGCCTGCGCAG
TCGAATGAGCGCTTAAAGCGCACGATGTCGATCAAAGCTCCGTGGGATAAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W4U0T2