Detailed information of TA system TA068514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 471095..471639
• Replicon: chromosome
• Accession: NZ_CP017999
• Organism: Rhizobium sp. Y9

Toxin (Protein)

• Gene name: parE
• Uniprot ID: U4PRC3
• Locus tag: BLX90_RS02310
• Protein ID: WP_004440407.1
• Coordinates: 471349..471639 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: BLX90_RS02305
• Protein ID: WP_004440406.1
• Coordinates: 471095..471346 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLX90_RS02275	466193..466882	-	690	WP_004440401.1	HAD family hydrolase	-
BLX90_RS02280	467097..467777	+	681	WP_004440402.1	GntR family transcriptional regulator	-
BLX90_RS02285	467818..468279	+	462	WP_034496277.1	DUF1284 domain-containing protein	-
BLX90_RS02290	468239..469339	-	1101	WP_034495932.1	hypothetical protein	-
BLX90_RS02300	469576..470721	+	1146	WP_004440405.1	site-specific DNA-methyltransferase	-
BLX90_RS02305	471095..471346	+	252	WP_004440406.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BLX90_RS02310	471349..471639	+	291	WP_004440407.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BLX90_RS02315	471671..472282	-	612	WP_004440408.1	HAD family phosphatase	-
BLX90_RS02320	472365..473474	-	1110	WP_004440409.1	A/G-specific adenine glycosylase	-
BLX90_RS25980	473387..473623	+	237	WP_162484560.1	hypothetical protein	-
BLX90_RS02325	473601..474065	+	465	WP_034495929.1	hypothetical protein	-
BLX90_RS02330	474093..474614	+	522	WP_004440412.1	DUF721 domain-containing protein	-
BLX90_RS02335	474743..475423	+	681	WP_004440414.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (2-79)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11253.77 Da
• Isoelectric Point: 6.6456

>T68514 WP_004440407.1 NZ_CP017999:471349-471639 [Rhizobium sp. Y9]
MGFRLSLPAEEDVIRIAQEGIDLFGPQQARKYHRELFAIFDLISRNPRIARERNELSPSVRIHPFRAHLVVYRVEADDDV
LIVRVRHGHEDWGNEP

Nucleotide

• Length: 291 bp

>T68514 NZ_CP017999:471349-471639 [Rhizobium sp. Y9]
ATGGGATTTCGCCTTTCTCTGCCTGCGGAAGAAGACGTCATCCGCATAGCGCAAGAAGGCATCGACCTATTTGGACCTCA
GCAAGCGCGAAAGTACCATCGCGAACTTTTCGCAATATTCGACTTGATATCACGGAATCCGAGGATCGCACGCGAGCGTA
ACGAGTTGTCGCCGTCGGTGCGCATTCATCCGTTCAGAGCACATCTGGTGGTCTATCGCGTTGAGGCGGACGACGACGTC
CTCATCGTCCGCGTCCGCCATGGGCACGAGGACTGGGGCAATGAGCCTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9281.45 Da
• Isoelectric Point: 6.4680

>AT68514 WP_004440406.1 NZ_CP017999:471095-471346 [Rhizobium sp. Y9]
MVTMNISLPGQMKEWVEAQSKTGRYSNASDYVRDLIRKDQERSEKIAAMQGLVDEGLQAPSGHRSEDALFAEAEARSMKH
KIG

Nucleotide

• Length: 252 bp

>AT68514 NZ_CP017999:471095-471346 [Rhizobium sp. Y9]
ATGGTAACCATGAATATTTCGCTGCCCGGCCAGATGAAGGAGTGGGTCGAAGCGCAGTCTAAAACGGGCCGTTACTCCAA
TGCCAGCGACTATGTACGAGATCTCATCCGAAAGGATCAGGAGCGGAGCGAAAAGATCGCTGCCATGCAGGGCCTGGTGG
ACGAAGGATTGCAGGCTCCCTCCGGTCATCGTTCTGAAGATGCCTTGTTTGCTGAGGCCGAAGCCCGCTCCATGAAACAT
AAAATTGGCTGA

Structures

Toxin

• AlphaFold DB: U4PRC3