Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4196834..4197453 Replicon chromosome
Accession NZ_CP017994
Organism Klebsiella pneumoniae strain P1428

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BMF28_RS20935 Protein ID WP_002892050.1
Coordinates 4196834..4197052 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BMF28_RS20940 Protein ID WP_002892066.1
Coordinates 4197079..4197453 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BMF28_RS20905 4192881..4193144 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
BMF28_RS20910 4193144..4193284 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BMF28_RS20915 4193281..4193979 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
BMF28_RS20920 4194080..4195531 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
BMF28_RS20925 4195506..4195976 - 471 WP_002892026.1 YlaC family protein -
BMF28_RS28590 4195997..4196137 + 141 WP_004147370.1 hypothetical protein -
BMF28_RS20930 4196109..4196675 - 567 WP_002892030.1 maltose O-acetyltransferase -
BMF28_RS20935 4196834..4197052 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BMF28_RS20940 4197079..4197453 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BMF28_RS20945 4197939..4201085 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
BMF28_RS20950 4201108..4202301 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T68512 WP_002892050.1 NZ_CP017994:c4197052-4196834 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T68512 NZ_CP017994:c4197052-4196834 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT68512 WP_002892066.1 NZ_CP017994:c4197453-4197079 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT68512 NZ_CP017994:c4197453-4197079 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References