Detailed information of TA system TA068505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1067653..1068323
• Replicon: chromosome
• Accession: NZ_CP017994
• Organism: Klebsiella pneumoniae strain P1428

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: BMF28_RS05535
• Protein ID: WP_004213072.1
• Coordinates: 1067880..1068323 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: BMF28_RS05530
• Protein ID: WP_004213073.1
• Coordinates: 1067653..1067883 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF28_RS05505	1063877..1064776	-	900	WP_004225022.1	nucleotide-binding protein	-
BMF28_RS05510	1064766..1065056	-	291	WP_004213078.1	hypothetical protein	-
BMF28_RS05515	1065408..1065614	+	207	WP_004213077.1	hypothetical protein	-
BMF28_RS05520	1065604..1065897	-	294	WP_004213076.1	hypothetical protein	-
BMF28_RS05525	1065913..1067045	-	1133	Protein_1055	DUF3800 domain-containing protein	-
BMF28_RS05530	1067653..1067883	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BMF28_RS05535	1067880..1068323	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
BMF28_RS05540	1068337..1068699	-	363	WP_198406734.1	hypothetical protein	-
BMF28_RS05545	1068756..1069049	-	294	Protein_1059	transposase	-
BMF28_RS28335	1069466..1070101	+	636	Protein_1060	response regulator	-
BMF28_RS05565	1070616..1071022	-	407	Protein_1061	GAF domain-containing protein	-
BMF28_RS05575	1071113..1072033	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
BMF28_RS05580	1072082..1072573	-	492	WP_011251327.1	DM13 domain-containing protein	-
BMF28_RS05585	1072636..1072911	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA / iucA / clbQ / clbP / clbO / clbN / clbM / clbL / clbK / clbJ / clbI / clbH / clbG / clbF / clbE / clbD / clbC / clbB / clbA	786952..1149612	362660
	-	flank	IS/Tn	-	-	1068678..1069049	371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T68505 WP_004213072.1 NZ_CP017994:1067880-1068323 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T68505 NZ_CP017994:1067880-1068323 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT68505 WP_004213073.1 NZ_CP017994:1067653-1067883 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT68505 NZ_CP017994:1067653-1067883 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620