Detailed information of TA system TA068491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5106298..5106914
• Replicon: chromosome
• Accession: NZ_CP017990
• Organism: Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WM95_RS25505
• Protein ID: WP_080298642.1
• Coordinates: 5106298..5106669 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1Z3N2D4
• Locus tag: WM95_RS25510
• Protein ID: WP_047743309.1
• Coordinates: 5106672..5106914 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM95_RS25490	5103798..5104700	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
WM95_RS25495	5104697..5105332	+	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
WM95_RS25500	5105329..5106258	+	930	WP_010436850.1	formate dehydrogenase accessory protein FdhE	-
WM95_RS25505	5106298..5106669	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
WM95_RS25510	5106672..5106914	-	243	WP_047743309.1	ribbon-helix-helix protein, CopG family	Antitoxin
WM95_RS25515	5107114..5108034	+	921	WP_063408691.1	alpha/beta hydrolase	-
WM95_RS25520	5108043..5108984	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
WM95_RS25525	5109029..5109466	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
WM95_RS25530	5109463..5110344	-	882	WP_023309756.1	virulence factor BrkB family protein	-
WM95_RS25535	5110338..5110937	-	600	WP_063408690.1	glucose-1-phosphatase	-
WM95_RS25540	5111058..5111858	-	801	WP_047174093.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T68491 WP_080298642.1 NZ_CP017990:c5106669-5106298 [Enterobacter cloacae complex sp. ECNIH7]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T68491 NZ_CP017990:c5106669-5106298 [Enterobacter cloacae complex sp. ECNIH7]
ATGGAGCACATGGCAGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATCGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CTAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9443.59 Da
• Isoelectric Point: 4.2917

>AT68491 WP_047743309.1 NZ_CP017990:c5106914-5106672 [Enterobacter cloacae complex sp. ECNIH7]
MSLSADLDMGRILIDLSDDVIQRLENLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT68491 NZ_CP017990:c5106914-5106672 [Enterobacter cloacae complex sp. ECNIH7]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGAAAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3N2D4