Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4446332..4447011 Replicon chromosome
Accession NZ_CP017990
Organism Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3N0J5
Locus tag WM95_RS22075 Protein ID WP_045268641.1
Coordinates 4446332..4446673 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A1Z3N0L6
Locus tag WM95_RS22080 Protein ID WP_045268640.1
Coordinates 4446694..4447011 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM95_RS22055 4443084..4444931 + 1848 WP_023309228.1 RNA polymerase sigma factor RpoD -
WM95_RS22065 4445463..4445969 - 507 WP_024906471.1 G/U mismatch-specific DNA glycosylase -
WM95_RS22075 4446332..4446673 - 342 WP_045268641.1 TA system toxin CbtA family protein Toxin
WM95_RS22080 4446694..4447011 - 318 WP_045268640.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
WM95_RS22085 4447031..4447252 - 222 WP_007893437.1 DUF987 domain-containing protein -
WM95_RS22090 4447261..4447737 - 477 WP_045268639.1 RadC family protein -
WM95_RS22095 4447753..4448211 - 459 WP_045268638.1 antirestriction protein -
WM95_RS27285 4448313..4448497 - 185 Protein_4249 DUF905 family protein -
WM95_RS22105 4448886..4450544 + 1659 WP_045268637.1 AAA family ATPase -
WM95_RS22110 4450537..4451691 + 1155 WP_045268636.1 UvrD-helicase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13045.30 Da        Isoelectric Point: 10.5786

>T68490 WP_045268641.1 NZ_CP017990:c4446673-4446332 [Enterobacter cloacae complex sp. ECNIH7]
MKTLPATTLRVVKPCLSPVAIWKMLLTRLLEQHYGLTLNDTPFSKERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Download         Length: 342 bp

>T68490 NZ_CP017990:c4446673-4446332 [Enterobacter cloacae complex sp. ECNIH7]
ATGAAAACTTTACCTGCAACAACTCTGCGGGTGGTGAAGCCCTGCCTGTCACCCGTGGCTATCTGGAAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTAAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGGTTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11976.67 Da        Isoelectric Point: 6.7379

>AT68490 WP_045268640.1 NZ_CP017990:c4447011-4446694 [Enterobacter cloacae complex sp. ECNIH7]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGMFSDVECLKLDDTFQHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT68490 NZ_CP017990:c4447011-4446694 [Enterobacter cloacae complex sp. ECNIH7]
ATGACCCACATCATCTGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGACT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGTCGAATGCCTGAAGCTGGATGACACATTCCAGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3N0J5

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3N0L6

References