Detailed information of TA system TA068476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 1894842..1895639
• Replicon: chromosome
• Accession: NZ_CP017990
• Organism: Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: WM95_RS09355
• Protein ID: WP_176600596.1
• Coordinates: 1895139..1895639 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: WM95_RS09350
• Protein ID: WP_023311079.1
• Coordinates: 1894842..1895111 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM95_RS09320	1890467..1890910	-	444	WP_047173168.1	helix-turn-helix transcriptional regulator	-
WM95_RS09325	1891063..1892304	+	1242	WP_063407990.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
WM95_RS09330	1892339..1892566	-	228	WP_008499832.1	YccJ family protein	-
WM95_RS09335	1892587..1893183	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
WM95_RS09340	1893575..1893745	+	171	WP_023311077.1	general stress protein	-
WM95_RS09345	1893863..1894780	+	918	WP_063407989.1	DMT family transporter	-
WM95_RS09350	1894842..1895111	+	270	WP_023311079.1	DUF1778 domain-containing protein	Antitoxin
WM95_RS09355	1895139..1895639	+	501	WP_176600596.1	GNAT family N-acetyltransferase	Toxin
WM95_RS09360	1895713..1897035	-	1323	WP_063407987.1	pyrimidine utilization transport protein G	-
WM95_RS09365	1897057..1897551	-	495	WP_063407986.1	pyrimidine utilization flavin reductase protein F	-
WM95_RS09370	1897561..1898151	-	591	WP_063407985.1	malonic semialdehyde reductase	-
WM95_RS09375	1898161..1898961	-	801	WP_063407984.1	pyrimidine utilization protein D	-
WM95_RS09380	1898969..1899355	-	387	WP_010429508.1	pyrimidine utilization protein C	-
WM95_RS09385	1899367..1900056	-	690	WP_023311085.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18989.66 Da
• Isoelectric Point: 7.9145

>T68476 WP_176600596.1 NZ_CP017990:1895139-1895639 [Enterobacter cloacae complex sp. ECNIH7]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDAFNERAKRFYLKQGFIPLTGENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T68476 NZ_CP017990:1895139-1895639 [Enterobacter cloacae complex sp. ECNIH7]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGTGTCCTGGGTTATT
ACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGGATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCTTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGGTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10249.72 Da
• Isoelectric Point: 5.8836

>AT68476 WP_023311079.1 NZ_CP017990:1894842-1895111 [Enterobacter cloacae complex sp. ECNIH7]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWTAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT68476 NZ_CP017990:1894842-1895111 [Enterobacter cloacae complex sp. ECNIH7]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGACTGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA