Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1178704..1179324 Replicon chromosome
Accession NZ_CP017990
Organism Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag WM95_RS05700 Protein ID WP_008499287.1
Coordinates 1178704..1178922 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag WM95_RS05705 Protein ID WP_008499288.1
Coordinates 1178950..1179324 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM95_RS05670 1174718..1174978 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
WM95_RS05675 1174981..1175121 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
WM95_RS05680 1175118..1175828 - 711 WP_023310596.1 GNAT family N-acetyltransferase -
WM95_RS05685 1175930..1177390 + 1461 WP_063409378.1 PLP-dependent aminotransferase family protein -
WM95_RS05690 1177362..1177829 - 468 WP_023310598.1 hypothetical protein -
WM95_RS05695 1177947..1178498 - 552 WP_023310599.1 maltose O-acetyltransferase -
WM95_RS05700 1178704..1178922 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
WM95_RS05705 1178950..1179324 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
WM95_RS05710 1179836..1182982 - 3147 WP_045356435.1 multidrug efflux RND transporter permease subunit -
WM95_RS05715 1183005..1184198 - 1194 WP_063409376.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T68475 WP_008499287.1 NZ_CP017990:c1178922-1178704 [Enterobacter cloacae complex sp. ECNIH7]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T68475 NZ_CP017990:c1178922-1178704 [Enterobacter cloacae complex sp. ECNIH7]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTCGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT68475 WP_008499288.1 NZ_CP017990:c1179324-1178950 [Enterobacter cloacae complex sp. ECNIH7]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT68475 NZ_CP017990:c1179324-1178950 [Enterobacter cloacae complex sp. ECNIH7]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References