Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 1013029..1013696 Replicon chromosome
Accession NZ_CP017990
Organism Enterobacter cloacae complex sp. ECNIH7

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3MRP6
Locus tag WM95_RS04860 Protein ID WP_001581456.1
Coordinates 1013029..1013358 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag WM95_RS04865 Protein ID WP_000070396.1
Coordinates 1013379..1013696 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM95_RS04845 1009662..1010651 + 990 WP_072262748.1 ABC transporter permease -
WM95_RS04850 1010740..1011690 + 951 WP_001581454.1 D-ribose ABC transporter substrate-binding protein -
WM95_RS04855 1011821..1012678 + 858 WP_001581455.1 DeoR/GlpR family DNA-binding transcription regulator -
WM95_RS04860 1013029..1013358 - 330 WP_001581456.1 toxin Toxin
WM95_RS04865 1013379..1013696 - 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
WM95_RS04870 1013715..1013936 - 222 WP_000691994.1 DUF987 domain-containing protein -
WM95_RS04875 1013945..1014421 - 477 WP_000811693.1 RadC family protein -
WM95_RS04880 1014437..1014895 - 459 WP_000211838.1 antirestriction protein -
WM95_RS04885 1014993..1015232 - 240 WP_000194654.1 DUF905 domain-containing protein -
WM95_RS04890 1015309..1015776 - 468 WP_001581459.1 hypothetical protein -
WM95_RS04895 1015800..1016243 - 444 WP_032199444.1 hypothetical protein -
WM95_RS04900 1016243..1016479 - 237 WP_001144031.1 hypothetical protein -
WM95_RS04905 1016520..1017221 - 702 WP_088544651.1 WYL domain-containing protein -
WM95_RS04910 1017438..1018259 - 822 WP_057061660.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12456.52 Da        Isoelectric Point: 8.5011

>T68474 WP_001581456.1 NZ_CP017990:c1013358-1013029 [Enterobacter cloacae complex sp. ECNIH7]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Download         Length: 330 bp

>T68474 NZ_CP017990:c1013358-1013029 [Enterobacter cloacae complex sp. ECNIH7]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAGACTCCGTATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGACTTGCTAAAAACTAA
CGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT68474 WP_000070396.1 NZ_CP017990:c1013696-1013379 [Enterobacter cloacae complex sp. ECNIH7]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT68474 NZ_CP017990:c1013696-1013379 [Enterobacter cloacae complex sp. ECNIH7]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MRP6

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References