Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47257..47900 Replicon plasmid unnamed4
Accession NZ_CP017989
Organism Klebsiella pneumoniae strain 825795-1

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB783_RS30545 Protein ID WP_040155119.1
Coordinates 47484..47900 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB783_RS30540 Protein ID WP_001261276.1
Coordinates 47257..47487 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB783_RS30495 42371..42640 + 270 WP_000339857.1 hypothetical protein -
BB783_RS30500 42817..43683 - 867 WP_004118283.1 replication initiation protein -
BB783_RS32725 44218..44322 + 105 WP_032409716.1 hypothetical protein -
BB783_RS30515 44451..44708 + 258 WP_000764642.1 hypothetical protein -
BB783_RS30520 44766..45542 - 777 WP_000015958.1 site-specific integrase -
BB783_RS30525 45539..46282 - 744 WP_000129823.1 hypothetical protein -
BB783_RS30530 46333..46683 - 351 WP_000493378.1 hypothetical protein -
BB783_RS30540 47257..47487 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB783_RS30545 47484..47900 + 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB783_RS30550 47941..48819 - 879 WP_009309916.1 restriction endonuclease -
BB783_RS30555 49481..50758 + 1278 Protein_55 HlyD family secretion protein -
BB783_RS30560 50748..51701 + 954 Protein_56 colicin V synthesis protein -
BB783_RS30570 51758..52455 + 698 Protein_57 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qacE / sul1 / qnrS1 / blaTEM-1B / blaCTX-M-15 / aph(6)-Id / aph(3'')-Ib / tet(A) / dfrA1 - 1..61011 61011
- inside Integron - - 223..60781 60558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T68472 WP_040155119.1 NZ_CP017989:47484-47900 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T68472 NZ_CP017989:47484-47900 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT68472 WP_001261276.1 NZ_CP017989:47257-47487 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT68472 NZ_CP017989:47257-47487 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References