Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5284054..5284679 Replicon chromosome
Accession NZ_CP017985
Organism Klebsiella pneumoniae strain 825795-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag BB783_RS27165 Protein ID WP_002882817.1
Coordinates 5284054..5284437 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag BB783_RS27170 Protein ID WP_004150355.1
Coordinates 5284437..5284679 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB783_RS27150 5281420..5282322 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
BB783_RS27155 5282319..5282954 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
BB783_RS27160 5282951..5283880 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
BB783_RS27165 5284054..5284437 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
BB783_RS27170 5284437..5284679 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
BB783_RS27175 5284884..5285801 + 918 WP_021466676.1 alpha/beta hydrolase -
BB783_RS27180 5285815..5286756 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
BB783_RS27185 5286801..5287238 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
BB783_RS27190 5287235..5288095 - 861 WP_004146232.1 virulence factor BrkB family protein -
BB783_RS27195 5288089..5288688 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T68468 WP_002882817.1 NZ_CP017985:c5284437-5284054 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T68468 NZ_CP017985:c5284437-5284054 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT68468 WP_004150355.1 NZ_CP017985:c5284679-5284437 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT68468 NZ_CP017985:c5284679-5284437 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References