68444

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4347906..4348501 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag BL257_RS22985 Protein ID WP_000239577.1
Coordinates 4348151..4348501 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag BL257_RS22980 Protein ID WP_001223208.1
Coordinates 4347906..4348157 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS22970 (BL257_21510) 4343571..4347350 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
BL257_RS22975 (BL257_21515) 4347353..4347694 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
BL257_RS22980 (BL257_21520) 4347906..4348157 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
BL257_RS22985 (BL257_21525) 4348151..4348501 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
BL257_RS22990 (BL257_21530) 4348581..4349111 - 531 WP_000055075.1 inorganic diphosphatase -
BL257_RS22995 (BL257_21535) 4349421..4350377 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
BL257_RS23000 (BL257_21540) 4350517..4352019 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
BL257_RS23005 (BL257_21545) 4352033..4353055 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T68444 WP_000239577.1 NZ_CP017979:4348151-4348501 [Escherichia coli str. K-12 substr. W3110]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T68444 NZ_CP017979:4348151-4348501 [Escherichia coli str. K-12 substr. W3110]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT68444 WP_001223208.1 NZ_CP017979:4347906-4348157 [Escherichia coli str. K-12 substr. W3110]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT68444 NZ_CP017979:4347906-4348157 [Escherichia coli str. K-12 substr. W3110]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References