Detailed information of TA system TA068439

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2948693..2949347
• Replicon: chromosome
• Accession: NZ_CP017979
• Organism: Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: BL257_RS15610
• Protein ID: WP_000244777.1
• Coordinates: 2948693..2949100 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: BL257_RS15615
• Protein ID: WP_000354046.1
• Coordinates: 2949081..2949347 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL257_RS15590 (BL257_14630)	2944650..2946383	-	1734	WP_000813200.1	single-stranded-DNA-specific exonuclease RecJ	-
BL257_RS15595 (BL257_14635)	2946389..2947099	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
BL257_RS15600 (BL257_14640)	2947124..2948020	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
BL257_RS15605 (BL257_14645)	2948132..2948653	+	522	WP_001055874.1	flavodoxin FldB	-
BL257_RS15610 (BL257_14650)	2948693..2949100	-	408	WP_000244777.1	toxin CptA	Toxin
BL257_RS15615 (BL257_14655)	2949081..2949347	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
BL257_RS15620 (BL257_14660)	2949590..2950570	+	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
BL257_RS15625 (BL257_14665)	2950766..2951425	-	660	WP_000250274.1	hemolysin III family protein	-
BL257_RS15630 (BL257_14670)	2951589..2951900	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
BL257_RS15635 (BL257_14675)	2951945..2953378	+	1434	WP_001336277.1	6-phospho-beta-glucosidase BglA	-
BL257_RS15640 (BL257_14680)	2953435..2954178	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T68439 WP_000244777.1 NZ_CP017979:c2949100-2948693 [Escherichia coli str. K-12 substr. W3110]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T68439 NZ_CP017979:c2949100-2948693 [Escherichia coli str. K-12 substr. W3110]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT68439 WP_000354046.1 NZ_CP017979:c2949347-2949081 [Escherichia coli str. K-12 substr. W3110]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT68439 NZ_CP017979:c2949347-2949081 [Escherichia coli str. K-12 substr. W3110]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7

Antitoxin

• AlphaFold DB: A0A7U9QD57