Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2948693..2949347 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BL257_RS15610 Protein ID WP_000244777.1
Coordinates 2948693..2949100 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BL257_RS15615 Protein ID WP_000354046.1
Coordinates 2949081..2949347 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS15590 (BL257_14630) 2944650..2946383 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
BL257_RS15595 (BL257_14635) 2946389..2947099 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BL257_RS15600 (BL257_14640) 2947124..2948020 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BL257_RS15605 (BL257_14645) 2948132..2948653 + 522 WP_001055874.1 flavodoxin FldB -
BL257_RS15610 (BL257_14650) 2948693..2949100 - 408 WP_000244777.1 toxin CptA Toxin
BL257_RS15615 (BL257_14655) 2949081..2949347 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BL257_RS15620 (BL257_14660) 2949590..2950570 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BL257_RS15625 (BL257_14665) 2950766..2951425 - 660 WP_000250274.1 hemolysin III family protein -
BL257_RS15630 (BL257_14670) 2951589..2951900 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BL257_RS15635 (BL257_14675) 2951945..2953378 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BL257_RS15640 (BL257_14680) 2953435..2954178 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T68439 WP_000244777.1 NZ_CP017979:c2949100-2948693 [Escherichia coli str. K-12 substr. W3110]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T68439 NZ_CP017979:c2949100-2948693 [Escherichia coli str. K-12 substr. W3110]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT68439 WP_000354046.1 NZ_CP017979:c2949347-2949081 [Escherichia coli str. K-12 substr. W3110]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT68439 NZ_CP017979:c2949347-2949081 [Escherichia coli str. K-12 substr. W3110]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References