68437

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2685392..2686059 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag BL257_RS14315 Protein ID WP_001094400.1
Coordinates 2685730..2686059 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag BL257_RS14310 Protein ID WP_000072690.1
Coordinates 2685392..2685709 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS14280 (BL257_13375) 2680444..2681453 - 1010 Protein_2578 arsenic transporter -
BL257_RS14285 (BL257_13380) 2681595..2683298 + 1704 WP_000896263.1 hypothetical protein -
BL257_RS14290 2683870..2684093 + 224 Protein_2580 DUF905 family protein -
BL257_RS14295 (BL257_13385) 2684196..2684654 + 459 WP_000211841.1 antirestriction protein -
BL257_RS14300 (BL257_13390) 2684663..2685145 + 483 WP_001407480.1 RadC family protein -
BL257_RS14305 2685154..2685354 + 201 WP_001395452.1 DUF987 domain-containing protein -
BL257_RS14310 (BL257_13395) 2685392..2685709 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BL257_RS14315 (BL257_13400) 2685730..2686059 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
BL257_RS14320 (BL257_13405) 2686423..2691003 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T68437 WP_001094400.1 NZ_CP017979:2685730-2686059 [Escherichia coli str. K-12 substr. W3110]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T68437 NZ_CP017979:2685730-2686059 [Escherichia coli str. K-12 substr. W3110]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT68437 WP_000072690.1 NZ_CP017979:2685392-2685709 [Escherichia coli str. K-12 substr. W3110]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT68437 NZ_CP017979:2685392-2685709 [Escherichia coli str. K-12 substr. W3110]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References