Detailed information of TA system TA068423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1413056..1413694
• Replicon: chromosome
• Accession: NZ_CP017979
• Organism: Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BL257_RS07620
• Protein ID: WP_000813794.1
• Coordinates: 1413056..1413232 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BL257_RS07625
• Protein ID: WP_001270286.1
• Coordinates: 1413278..1413694 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL257_RS07600 (BL257_07090)	1408675..1409889	-	1215	WP_001326689.1	BenE family transporter YdcO	-
BL257_RS07605 (BL257_07095)	1409942..1410478	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
BL257_RS07610 (BL257_07100)	1410551..1412512	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BL257_RS07615 (BL257_07105)	1412604..1412834	-	231	WP_000494244.1	YncJ family protein	-
BL257_RS07620 (BL257_07110)	1413056..1413232	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BL257_RS07625 (BL257_07115)	1413278..1413694	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BL257_RS07630 (BL257_07120)	1413773..1415179	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
BL257_RS07635 (BL257_07125)	1415424..1416569	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
BL257_RS07640 (BL257_07130)	1416587..1417600	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
BL257_RS07645 (BL257_07135)	1417601..1418542	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T68423 WP_000813794.1 NZ_CP017979:1413056-1413232 [Escherichia coli str. K-12 substr. W3110]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T68423 NZ_CP017979:1413056-1413232 [Escherichia coli str. K-12 substr. W3110]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT68423 WP_001270286.1 NZ_CP017979:1413278-1413694 [Escherichia coli str. K-12 substr. W3110]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT68423 NZ_CP017979:1413278-1413694 [Escherichia coli str. K-12 substr. W3110]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB