68418

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 382073..382691 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BL257_RS02045 Protein ID WP_001291435.1
Coordinates 382073..382291 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BL257_RS02050 Protein ID WP_000344800.1
Coordinates 382317..382691 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS02010 (BL257_01865) 377362..377934 + 573 WP_000779842.1 YbaY family lipoprotein -
BL257_RS02015 (BL257_01870) 377965..378276 - 312 WP_000409911.1 MGMT family protein -
BL257_RS02025 (BL257_01880) 378655..379008 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BL257_RS02030 (BL257_01885) 379050..380600 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BL257_RS02035 (BL257_01890) 380764..381234 - 471 WP_000136192.1 YlaC family protein -
BL257_RS02040 (BL257_01895) 381350..381901 - 552 WP_000102564.1 maltose O-acetyltransferase -
BL257_RS02045 (BL257_01900) 382073..382291 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BL257_RS02050 (BL257_01905) 382317..382691 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BL257_RS02055 (BL257_01910) 383237..386386 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BL257_RS02060 (BL257_01915) 386409..387602 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T68418 WP_001291435.1 NZ_CP017979:c382291-382073 [Escherichia coli str. K-12 substr. W3110]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T68418 NZ_CP017979:c382291-382073 [Escherichia coli str. K-12 substr. W3110]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT68418 WP_000344800.1 NZ_CP017979:c382691-382317 [Escherichia coli str. K-12 substr. W3110]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT68418 NZ_CP017979:c382691-382317 [Escherichia coli str. K-12 substr. W3110]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References