Detailed information of TA system TA068414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6617290..6617795
• Replicon: chromosome
• Accession: NZ_CP017969
• Organism: Pseudomonas aeruginosa isolate B10W

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: BMR72_RS31725
• Protein ID: WP_003083773.1
• Coordinates: 6617290..6617571 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: BMR72_RS31730
• Protein ID: WP_003083775.1
• Coordinates: 6617568..6617795 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMR72_RS31700	6612541..6613890	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
BMR72_RS31705	6613939..6614625	+	687	WP_003083762.1	FadR family transcriptional regulator	-
BMR72_RS31710	6614726..6615460	+	735	WP_003128333.1	GntR family transcriptional regulator	-
BMR72_RS31715	6615664..6616050	+	387	WP_003083767.1	aegerolysin family protein	-
BMR72_RS31720	6616082..6616990	-	909	WP_003083769.1	LysR family transcriptional regulator	-
BMR72_RS31725	6617290..6617571	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
BMR72_RS31730	6617568..6617795	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
BMR72_RS31735	6617971..6618591	-	621	WP_003101226.1	hypothetical protein	-
BMR72_RS31740	6618692..6619192	+	501	WP_003128336.1	LEA type 2 family protein	-
BMR72_RS31745	6619265..6619606	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
BMR72_RS31750	6619688..6621115	-	1428	WP_003083784.1	GABA permease	-
BMR72_RS31755	6621284..6622777	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T68414 WP_003083773.1 NZ_CP017969:c6617571-6617290 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T68414 NZ_CP017969:c6617571-6617290 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT68414 WP_003083775.1 NZ_CP017969:c6617795-6617568 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT68414 NZ_CP017969:c6617795-6617568 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9