68412

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4903326..4903934 Replicon chromosome
Accession NZ_CP017969
Organism Pseudomonas aeruginosa isolate B10W

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag BMR72_RS23755 Protein ID WP_003114156.1
Coordinates 4903326..4903673 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag BMR72_RS23760 Protein ID WP_003114155.1
Coordinates 4903683..4903934 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BMR72_RS23725 4898685..4898957 + 273 WP_003134390.1 cysteine-rich CWC family protein -
BMR72_RS23730 4898957..4899649 + 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
BMR72_RS23735 4899785..4900828 + 1044 WP_003134392.1 L,D-transpeptidase -
BMR72_RS23740 4900908..4901645 + 738 WP_003124252.1 murein L,D-transpeptidase catalytic domain family protein -
BMR72_RS23745 4902097..4902999 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
BMR72_RS23755 4903326..4903673 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BMR72_RS23760 4903683..4903934 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BMR72_RS23765 4904148..4905130 - 983 Protein_4582 tyrosine-type recombinase/integrase -
BMR72_RS23770 4905130..4906422 - 1293 WP_003134394.1 hypothetical protein -
BMR72_RS23775 4906652..4907935 - 1284 WP_023127773.1 hypothetical protein -
BMR72_RS23780 4907939..4908295 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4903326..4929289 25963


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T68412 WP_003114156.1 NZ_CP017969:c4903673-4903326 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T68412 NZ_CP017969:c4903673-4903326 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT68412 WP_003114155.1 NZ_CP017969:c4903934-4903683 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT68412 NZ_CP017969:c4903934-4903683 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References