Detailed information of TA system TA068405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1600510..1601118
• Replicon: chromosome
• Accession: NZ_CP017968
• Organism: Paenibacillus polymyxa strain YC0573

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: PPYC2_RS07135
• Protein ID: WP_007429335.1
• Coordinates: 1600768..1601118 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PPYC2_RS07130
• Protein ID: WP_170970806.1
• Coordinates: 1600510..1600761 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPYC2_RS07115	1596711..1597343	+	633	WP_023987593.1	LysE family transporter	-
PPYC2_RS07120	1597454..1598731	+	1278	WP_071559019.1	outer membrane lipoprotein carrier protein LolA	-
PPYC2_RS07125	1598990..1600186	+	1197	WP_028543050.1	alanine racemase	-
PPYC2_RS07130	1600510..1600761	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
PPYC2_RS07135	1600768..1601118	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PPYC2_RS07140	1601325..1603520	+	2196	WP_179085433.1	RNA-binding transcriptional accessory protein	-
PPYC2_RS07145	1603625..1603759	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
PPYC2_RS07150	1603904..1604440	+	537	WP_034094995.1	SprT family protein	-
PPYC2_RS07165	1604829..1605704	-	876	WP_023987597.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T68405 WP_007429335.1 NZ_CP017968:1600768-1601118 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T68405 NZ_CP017968:1600768-1601118 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCTATCACCGCTCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTAGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT68405 WP_170970806.1 NZ_CP017968:1600510-1600761 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT68405 NZ_CP017968:1600510-1600761 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATCAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1