Detailed information of TA system TA068404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1517041..1517649
• Replicon: chromosome
• Accession: NZ_CP017967
• Organism: Paenibacillus polymyxa strain YC0136

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: PPYC1_RS06495
• Protein ID: WP_007429335.1
• Coordinates: 1517299..1517649 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PPYC1_RS06490
• Protein ID: WP_170970806.1
• Coordinates: 1517041..1517292 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPYC1_RS06475	1513242..1513874	+	633	WP_071639936.1	LysE family transporter	-
PPYC1_RS06480	1513985..1515262	+	1278	WP_071639937.1	outer membrane lipoprotein carrier protein LolA	-
PPYC1_RS06485	1515521..1516717	+	1197	WP_071639938.1	alanine racemase	-
PPYC1_RS06490	1517041..1517292	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
PPYC1_RS06495	1517299..1517649	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PPYC1_RS06500	1517829..1520051	+	2223	WP_179085886.1	RNA-binding transcriptional accessory protein	-
PPYC1_RS06505	1520156..1520290	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
PPYC1_RS06510	1520435..1520947	+	513	WP_071639939.1	SprT family protein	-
PPYC1_RS06525	1521337..1522212	-	876	WP_071639940.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T68404 WP_007429335.1 NZ_CP017967:1517299-1517649 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T68404 NZ_CP017967:1517299-1517649 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCCATCACCGCCCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTAGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT68404 WP_170970806.1 NZ_CP017967:1517041-1517292 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT68404 NZ_CP017967:1517041-1517292 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1