Detailed information of TA system TA068401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 445444..446092
• Replicon: chromosome
• Accession: NZ_CP017962
• Organism: Virgibacillus halodenitrificans strain PDB-F2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6L8JWB3
• Locus tag: BME96_RS02370
• Protein ID: WP_019379217.1
• Coordinates: 445733..446092 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BME96_RS02365
• Protein ID: WP_019379218.1
• Coordinates: 445444..445728 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME96_RS02345	440929..441288	+	360	WP_071648183.1	holo-ACP synthase	-
BME96_RS02350	441348..442868	+	1521	WP_071648184.1	NAD(P)H-hydrate dehydratase	-
BME96_RS02355	442956..443966	+	1011	WP_071648185.1	outer membrane lipoprotein carrier protein LolA	-
BME96_RS02360	444157..445281	+	1125	WP_071648186.1	alanine racemase	-
BME96_RS02365	445444..445728	+	285	WP_019379218.1	hypothetical protein	Antitoxin
BME96_RS02370	445733..446092	+	360	WP_019379217.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BME96_RS02375	446439..447308	+	870	WP_060681896.1	RsbT co-antagonist protein RsbRA	-
BME96_RS02380	447314..447670	+	357	WP_040954281.1	STAS domain-containing protein	-
BME96_RS02385	447673..448074	+	402	WP_060681897.1	anti-sigma regulatory factor	-
BME96_RS02390	448181..449194	+	1014	WP_060681898.1	PP2C family protein-serine/threonine phosphatase	-
BME96_RS02395	449299..449631	+	333	WP_060681899.1	STAS domain-containing protein	-
BME96_RS02400	449631..450107	+	477	WP_019379211.1	anti-sigma B factor RsbW	-
BME96_RS02405	450079..450870	+	792	WP_071648187.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13377.53 Da
• Isoelectric Point: 8.4940

>T68401 WP_019379217.1 NZ_CP017962:445733-446092 [Virgibacillus halodenitrificans]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDAEMMNKINQALEISLGLKDMYDH

Nucleotide

• Length: 360 bp

>T68401 NZ_CP017962:445733-446092 [Virgibacillus halodenitrificans]
TTGATCGTTCAAAGAGGTGAAGTATATTTCGCCGACTTATCCCCTGTGGTTGGATCGGAGCAGGGAGGCGTGCGCCCGGT
ATTGATCCTTCAAAATGATATAGGTAATCGGTTTAGCCCGACCGTTATTGTGGCAGCTATTACTGCGCAAATTCAAAAGG
CTAAGTTACCTACTCATGTTGAAATTAATGCAAAACGCTACGGATTCGACCGGAATTCTGTTATTCTGCTGGAACAAATC
CGTACATTGGATAAACAGAGATTAACAGATAAAATAACGAAACTTGATGCTGAAATGATGAATAAAATAAATCAGGCTCT
GGAAATTAGTCTGGGGCTGAAAGATATGTATGATCATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10910.24 Da
• Isoelectric Point: 4.3735

>AT68401 WP_019379218.1 NZ_CP017962:445444-445728 [Virgibacillus halodenitrificans]
MSESLQEILVKIPKNLLNEVDGLMRYENSDLSDFICQATRSYLQHKKDEHIQQFRETMQKGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT68401 NZ_CP017962:445444-445728 [Virgibacillus halodenitrificans]
TTGTCAGAGAGCTTACAGGAGATCTTAGTGAAAATACCAAAAAACCTATTGAATGAGGTGGATGGATTAATGAGATATGA
AAATAGTGACTTAAGTGATTTCATTTGTCAGGCTACAAGAAGCTACTTGCAGCATAAAAAAGACGAACATATCCAACAAT
TCCGTGAGACAATGCAAAAAGGCTATGAAGAAATGGGTCGCATTAATCTAACAATTGCTTCGGAAGCTTTTCAAGCTGAA
GAGGAGGCTGAAAACACCTTAGAGCGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A6L8JWB3