Detailed information of TA system TA068400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 959803..961447
• Replicon: chromosome
• Accession: NZ_CP017961
• Organism: Pasteurella multocida strain Razi_Pm0001

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V4NI19
• Locus tag: BMF22_RS04715
• Protein ID: WP_005752891.1
• Coordinates: 959803..961110 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: BMF22_RS04720
• Protein ID: WP_005752888.1
• Coordinates: 961112..961447 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF22_RS04705	955801..958383	+	2583	WP_005752311.1	DNA mismatch repair protein MutS	-
BMF22_RS04710	958449..959585	-	1137	WP_005752312.1	anhydro-N-acetylmuramic acid kinase	-
BMF22_RS04715	959803..961110	+	1308	WP_005752891.1	HipA domain-containing protein	Toxin
BMF22_RS04720	961112..961447	+	336	WP_005752888.1	helix-turn-helix domain-containing protein	Antitoxin
BMF22_RS04725	961543..962211	-	669	WP_005752314.1	NAD(P)H-dependent oxidoreductase	-
BMF22_RS04730	962252..963618	-	1367	Protein_916	oxygen-independent coproporphyrinogen III oxidase	-
BMF22_RS04735	963642..964070	-	429	WP_005752316.1	DUF2489 domain-containing protein	-
BMF22_RS04740	964076..964645	-	570	WP_005752317.1	Der GTPase-activating protein YihI	-
BMF22_RS04745	964709..965368	-	660	WP_005752318.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50526.31 Da
• Isoelectric Point: 4.9118

>T68400 WP_005752891.1 NZ_CP017961:959803-961110 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T68400 NZ_CP017961:959803-961110 [Pasteurella multocida]
ATGGAAAAACTTACACTGCAAATTTATCTTGAACAAGCTTGGTGCGATTTAGCTTTCTTGTATTTTGATTCAGAAACCTA
TCAACTGTGTAATTTCGAATACTTAACAGATTACGTGGTTCAGCACTATGGAAAAACGGGTGAACATGCTGTGTCGCTTA
ATTATCCAGTGAATGTTTTCATTGATGACACGACGTCGCTGTGGAGCTGGCTCAGTGATATCGCGCCTGCGGGAGCAAGT
CGTCGTTATTGGTTAGAAGCGTTAGACATTAAACAAGAATCAGAGGAAAAACAACAGTTTCTTTTACTGAAACATGCTGC
GATTTCCCCAATTGGCAATTTACGTATCAAAGATGCTGTGCCAGAACAAGTTGAAGTTCGCTTATTTGATCTAGACAAGG
CGAAAGATAGACAGAGTGATTTTTTAGAGTATGTGAACCAAAGTGGTGCGATGGTAGGAGGTGCGACAGGGGCTGGCGGC
GAAGCACCAAAATTGCTTCTTAAACGGCGAGAGAATCAAGTTTGGATTGATAACCAACAGCAAGGGCAGGATCAGGATGT
GAGTTATTTAGTGAAGTACCCACGTGGCAATTACTCACCCATTGATTGTGATATTTTACGTGCAGAATATCATTATTATC
AGGAACTTGCTCAACTGGGTTTTGACACAATAGATACGACTAGAATGAGGTTAGAAGAAGGAAAACGTTATCCTTCCCTA
TGGTTGCCTCGTTTTGATATTGTGCAACGAGAAGGAAAAACACATCGTCTTGCTTTTGAATCAGTGTATTCGCTATTACA
GAAACAAGGTGGTGTACTTGATCATGAAACGACACTTCGCCAGTTAATTGAGAGAATCTCGACGGCTCGTTTGCAGTTTG
CACAAACAGACTTCGTGATTGAATGGGTAAAGCGAGATTTGTTAAATATTGCCTTTGGCAATAGCGACAATCATGGTCGT
AATATGGCGTTTTTACGTGATGAACAACGTATTTGGCTCGCCCCAATTTATGATTTTGCGCCGATGCGTGCTGATCCAGA
AGGCATTATTCGTACGACTACTTGGCAAACTAAAGGGGAGTATCCACAGGAATTTGCTGGCGAGTATCACTTTGAGCGCA
TCGCAGAAGTATTAGCTGATTTAGTTGAACCAACGTTATTAATGCAAGAGTTACGCCGGTTAGCTGGCAGTTTAGTGGAT
TTAAAATCCCGATTAGTACAACGTAATCTACCTCAATCTATTTTAGATTTCCCTGCGATTGGTTTTGATTATTTACCTGA
AAAATTAAAAAAATGGGGGCTTATTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT68400 WP_005752888.1 NZ_CP017961:961112-961447 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT68400 NZ_CP017961:961112-961447 [Pasteurella multocida]
ATGCAAATCACTCGAAGAAATGCCTCTACCAAAGGGGCAAGTGTTGCGGAAATTGATCGTAAGAAAGTACGTAATGAAAT
CATTCAAGCGATGCTAATGGGGGAATTGAGCCAAGGTGAAGCATTGCGTAAATTGCGTATTGAAATGTTCGGTATTAATC
AACAACACTATATTCAGTTAGTAAAGGTATCACGCCAGACCCTATCGAATATTGAGAATGATAAGGGTAATTATAGTGTA
GAAACGATTAATAAAGTGTTTAAACCCTTTGGTCTGGAACTCGGCTTGGTACCTGTTTCTAAGGCATTATTAAAGGAACT
TTTTTCGACAGCTTAA

Structures

Toxin

• AlphaFold DB: V4NI19

Antitoxin

• AlphaFold DB: V4NAB0