Detailed information of TA system TA068399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 16768..17369
• Replicon: plasmid unnamed5
• Accession: NZ_CP017959
• Organism: Lactiplantibacillus plantarum strain C410L1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: BL295_RS16130
• Protein ID: WP_001748110.1
• Coordinates: 17025..17369 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: Q684P2
• Locus tag: BL295_RS16125
• Protein ID: WP_003643337.1
• Coordinates: 16768..17031 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL295_RS16090	12022..13197	-	1176	WP_071543008.1	IS256 family transposase	-
BL295_RS17225	13370..14068	+	699	WP_071543009.1	RepA	-
BL295_RS16100	14224..14421	+	198	WP_071543010.1	hypothetical protein	-
BL295_RS16105	14472..15218	+	747	WP_071543013.1	Fic family protein	-
BL295_RS16110	15341..15619	-	279	WP_013855167.1	hypothetical protein	-
BL295_RS16115	15639..15848	-	210	WP_050894606.1	hypothetical protein	-
BL295_RS17230	16095..16307	-	213	WP_174255630.1	integrase	-
BL295_RS17235	16314..16682	-	369	WP_187337832.1	hypothetical protein	-
BL295_RS16125	16768..17031	+	264	WP_003643337.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BL295_RS16130	17025..17369	+	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BL295_RS16135	17717..18007	-	291	WP_063207355.1	hypothetical protein	-
BL295_RS16140	18000..18278	-	279	WP_015063546.1	DUF2089 family protein	-
BL295_RS16145	18523..19286	-	764	Protein_27	helix-turn-helix transcriptional regulator	-
BL295_RS16150	19472..19684	-	213	WP_015063544.1	DUF3923 family protein	-
BL295_RS16155	19923..20411	+	489	WP_071543001.1	DUF536 domain-containing protein	-
BL295_RS16160	21116..21493	+	378	WP_071543002.1	YxeA family protein	-
BL295_RS16165	21638..22163	-	526	Protein_31	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56791	56791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T68399 WP_001748110.1 NZ_CP017959:17025-17369 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T68399 NZ_CP017959:17025-17369 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACGGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.51 Da
• Isoelectric Point: 6.4708

>AT68399 WP_003643337.1 NZ_CP017959:16768-17031 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT68399 NZ_CP017959:16768-17031 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A6L4ZZT2