Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 822..1423 Replicon plasmid unnamed5
Accession NZ_CP017959
Organism Lactiplantibacillus plantarum strain C410L1

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag BL295_RS16025 Protein ID WP_001748110.1
Coordinates 1079..1423 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag BL295_RS16020 Protein ID WP_003643337.1
Coordinates 822..1085 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL295_RS17215 149..361 - 213 WP_174255630.1 integrase -
BL295_RS17220 368..736 - 369 WP_187337832.1 hypothetical protein -
BL295_RS16020 822..1085 + 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BL295_RS16025 1079..1423 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BL295_RS16030 1771..2061 - 291 WP_063207355.1 hypothetical protein -
BL295_RS16035 2054..2332 - 279 WP_015063546.1 DUF2089 family protein -
BL295_RS16040 2577..3340 - 764 Protein_6 helix-turn-helix transcriptional regulator -
BL295_RS16045 3526..3738 - 213 WP_015063544.1 DUF3923 family protein -
BL295_RS16050 3977..4465 + 489 WP_071543001.1 DUF536 domain-containing protein -
BL295_RS16055 5170..5547 + 378 WP_071543002.1 YxeA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..56791 56791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T68398 WP_001748110.1 NZ_CP017959:1079-1423 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T68398 NZ_CP017959:1079-1423 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACGGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT68398 WP_003643337.1 NZ_CP017959:822-1085 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT68398 NZ_CP017959:822-1085 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References