Detailed information of TA system TA068396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 475501..476138
• Replicon: chromosome
• Accession: NZ_CP017953
• Organism: Bacillus amyloliquefaciens strain Y14

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAMY_RS02480
• Protein ID: WP_003156187.1
• Coordinates: 475788..476138 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAMY_RS02475
• Protein ID: WP_003156188.1
• Coordinates: 475501..475782 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMY_RS02455	471867..472466	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BAMY_RS02460	472559..472924	+	366	WP_014304402.1	holo-ACP synthase	-
BAMY_RS02465	473088..474095	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
BAMY_RS02470	474212..475381	+	1170	WP_025853726.1	alanine racemase	-
BAMY_RS02475	475501..475782	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAMY_RS02480	475788..476138	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAMY_RS02485	476256..477077	+	822	WP_003156182.1	STAS domain-containing protein	-
BAMY_RS02490	477082..477447	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAMY_RS02495	477450..477851	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAMY_RS02500	477863..478870	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
BAMY_RS02505	478934..479263	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BAMY_RS02510	479260..479742	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BAMY_RS02515	479708..480496	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BAMY_RS02520	480496..481098	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68396 WP_003156187.1 NZ_CP017953:475788-476138 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68396 NZ_CP017953:475788-476138 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68396 WP_003156188.1 NZ_CP017953:475501-475782 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68396 NZ_CP017953:475501-475782 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5